Release of recombinant human bone morphogenetic protein-2 from heterocyclic methacrylate polymer systems

• Published in: Biomaterials Vol. 22; no. 15; pp. 2081 - 2086
• Main Authors: Patel, M.P, Pavlovic, P, Hughes, F.J, King, G.N, Cruchley, A, Braden, M
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-08-2001; Elsevier Science
• Subjects: Biocompatible Materials - chemistry; Biological and medical sciences; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins - metabolism; Diffusion; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics; Humans; Kinetics; Maxillofacial surgery. Dental surgery. Orthodontics; Medical sciences; Methacrylate polymers; Methacrylates - chemistry; Polymers - chemistry; Protein Binding; Protein release; Recombinant Proteins - metabolism; rhBMP-2; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Temperature; Time Factors; Transforming Growth Factor beta; Water absorption; rhBMP-2; Water absorption; Methacrylate polymers; Diffusion; Protein release; Human; Biological properties; Animal model; Rat; Rodentia; Dentistry; Experimental study; Tetrahydrofuran polymer; Hydroxyethyl methacrylate polymer; Vertebrata; Mammalia; Bone morphogenetic protein; Animal; Reaction medium; Biomaterial; Osteogenesis; Ethyl acrylate polymer
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(00)00396-3

The release of recombinant human bone morphogenetic protein-2 (rhBMP-2) from three room temperature polymerising methacrylate systems has been studied. These all contained poly(ethyl methacrylate) powder, but the monomer liquids comprised, respectively, tetrahydrofurfuryl methacrylate (THFM), 90/10 THFM/hydroxyethyl methacrylate (HEMA), and 70/30 THFM/HEMA. In all cases, rhBMP-2 was released, but the addition of 10% HEMA accelerated release (a nine-fold increase in diffusion coefficient); a further increase to 30% HEMA had no additional effect. For most of the release process, a diffusion process operated, although the early stages were not well defined. At the end of the 15 day period, the release, respectively, for the PEM/THFM, PEM:90/10 THFM/HEMA and PEM:70/30 THFM/HEMA systems was 596, 878 and 923
ng (i.e. up to 92% of the rhBMP-2 added).