Vaccination of chickens with a temperature-sensitive mutant of Salmonella enteritidis

• Published in: Vaccine Vol. 18; no. 11; pp. 1140 - 1145
• Main Authors: Cerquetti, M.Cristina, Gherardi, M.Magdalena
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 06-01-2000; Elsevier
• Subjects: Animals; Bacterial Vaccines - immunology; Bacteriology; Biological and medical sciences; Chickens; Fundamental and applied biological sciences. Psychology; Live vaccine; Male; Microbiology; Mutation; Salmonella enteritidis; Salmonella enteritidis - immunology; Temperature; Temperature-sensitive; Vaccination; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Attenuated - immunology; Salmonella enteritidis; Live vaccine; Chickens; Temperature-sensitive; Immunoprotection; Booster vaccination; Toxicity; Vaccine; Route of administration; Dose activity relation; Vertebrata; Bacteria; Chicken; Aves; Veterinary; Attenuated strain; Enterobacteriaceae; Temperature sensitive mutation
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(99)00377-1

One-day old chickens were inoculated with temperature-sensitive mutant E/1/3 of
S. enteritidis. Two routes of inoculation were used: oral and intraperitoneal (ip). One group of chickens were given two oral inoculations (oral–oral). A second group received two ip inoculations (ip–ip). A third group received the first dose orally and the second ip (oral–ip) and the fourth group was given the first dose ip and the second dose orally (ip–oral). The vaccine strain was safe even when inoculated at high doses, and induced strong protection against virulent
S. enteritidis strain after oral challenge. Results show that vaccination with mutant E/1/3 reduced the number of animals shedding the pathogen after challenge. Furthermore, animals immunized oral–oral and oral–ip showed a significant reduction in cecal and spleen colonization by virulent
Salmonella.