Release of T-antigen, a carcinoma marker from native human cells, by endo-alpha-N-acetylgalactosaminidase of Alcaligenes sp

• Published in: Biochemical and biophysical research communications Vol. 172; no. 1; p. 341
• Main Authors: Fan, J Q, Kanatani, K, Andoh, N, Yamamoto, K, Kumagai, H, Tochikura, T
• Format: Journal Article
• Language: English
• Published: United States 15-10-1990
• Subjects: Alcaligenes - enzymology; alpha-N-Acetylgalactosaminidase; Antigens, Tumor-Associated, Carbohydrate; Biomarkers, Tumor - analysis; Cell Line; Chromatography, Affinity; Disaccharides - analysis; Erythrocytes - immunology; Glycophorin - isolation & purification; Hexosaminidases - metabolism; Humans; Kinetics; Stomach Neoplasms
• ISSN: 0006-291X
• DOI: 10.1016/S0006-291X(05)80215-3

The endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. could release T-antigen (Ga1 beta 1---3GalNAc), a carcinoma-associated marker from asialo glycophorin and human cells. The released T-antigen from human asialo erythrocytes was determined by thin layer chromatography and gas liquid chromatography. The released T-antigen from human gastric carcinoma cell Kato III was identified by high performance liquid chromatographies with a reverse-phase column and a size fractionation column. Released T-antigen could be analyzed quantitatively by a sensitive method including pyridylamino derivatization and following high performance liquid chromatography. This suggests that the enzyme is useful for detection and determination of T-antigen from cells.