Effects of metabolites of benzo(a)pyrene on unschedule DNA synthesis in BALB/3T3 cell line

• Published in: Chemosphere (Oxford) Vol. 41; no. 1; pp. 139 - 142
• Main Authors: Chen, J.K., Wu, Z.L., Liu, Y.G., Lei, Y.X.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01-07-2000; Elsevier
• Subjects: 3T3 Cells; Animals; benzo(a)pyrene; Benzo(a)pyrene - adverse effects; Benzo(a)pyrene - analogs & derivatives; Benzo(a)pyrene - pharmacology; Biological and medical sciences; Carcinogens - adverse effects; Carcinogens - pharmacology; Cell Culture Techniques; Cell Survival; Chemical and industrial products toxicology. Toxic occupational diseases; DNA - biosynthesis; DNA - drug effects; Dose-Response Relationship, Drug; Lung Neoplasms - chemically induced; Medical sciences; Mice; Mice, Inbred BALB C; Toxicity Tests; Toxicology; Various organic compounds; Carcinogen; Metabolite; Toxicity; Polycyclic aromatic compound; Mechanism of action; Unscheduled DNA synthesis; In vitro
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/S0045-6535(99)00401-4

In order to explore the damage from metabolites of benzo(a)pyrene on DNA of mammalian cells, the effects of four metabolites of benzo(a)pyrene (anti-BPDE, syn-BPDE, 3-OH-BP and 9-OH-BP) on synthesis of DNA and unschedule DNA synthesis (UDS) in BALB/3T3 cells were assayed, by methods of single-labeling and double-labeling. The results showed that all of the four agents were able to increase the synthesis of DNA, but only three of them (apart from syn-BPDE) induced UDS in BALB/3T3 cells. The above indicates that the metabolites of benzo(a)pyrene are able to damage DNA in BALB/3T3 cells, and that this effect may be relative to the sterical structure of metabolites of benzo(a)pyrene.