Metabolic functions of glycosomes in trypanosomatids

• Published in: Biochimica et biophysica acta Vol. 1763; no. 12; pp. 1463 - 1477
• Main Authors: Michels, Paul A.M., Bringaud, Frédéric, Herman, Murielle, Hannaert, Véronique
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-12-2006; Elsevier
• Subjects: Animals; Cell Compartmentation; Cell Differentiation; Differentiation; Glycolysis; Glycosome; Leishmania; Leishmania - metabolism; Life Sciences; Metabolic adaptation; Microbiology and Parasitology; Microbodies; Microbodies - metabolism; Mitochondria; Mitochondria - metabolism; Organelle turnover; Protozoan Proteins; Protozoan Proteins - metabolism; Trypanosoma; Trypanosoma - metabolism; Trypanosoma brucei brucei; Trypanosoma brucei brucei - metabolism; Trypanosome; FH; PAT; TAO; GPO; Gly3P; PTS; PFK; Metabolic adaptation; Trypanosome; PPDK; PYK; PEPCK; DHAP; MCF; TCA; ATG; PGK; Glycolysis; FRD; Glycosome; HXK; PEP; GAT; Differentiation; Organelle turnover
• ISSN: 0167-4889; 0006-3002; 1879-2596
• DOI: 10.1016/j.bbamcr.2006.08.019

Protozoan Kinetoplastida, including the pathogenic trypanosomatids of the genera
Trypanosoma and
Leishmania, compartmentalize several important metabolic systems in their peroxisomes which are designated glycosomes. The enzymatic content of these organelles may vary considerably during the life-cycle of most trypanosomatid parasites which often are transmitted between their mammalian hosts by insects. The glycosomes of the
Trypanosoma brucei form living in the mammalian bloodstream display the highest level of specialization; 90% of their protein content is made up of glycolytic enzymes. The compartmentation of glycolysis in these organelles appears essential for the regulation of this process and enables the cells to overcome short periods of anaerobiosis. Glycosomes of all other trypanosomatid forms studied contain an extended glycolytic pathway catalyzing the aerobic fermentation of glucose to succinate. In addition, these organelles contain enzymes for several other processes such as the pentose-phosphate pathway, β-oxidation of fatty acids, purine salvage, and biosynthetic pathways for pyrimidines, ether-lipids and squalenes. The enzymatic content of glycosomes is rapidly changed during differentiation of mammalian bloodstream-form trypanosomes to the forms living in the insect midgut. Autophagy appears to play an important role in trypanosomatid differentiation, and several lines of evidence indicate that it is then also involved in the degradation of old glycosomes, while a population of new organelles containing different enzymes is synthesized. The compartmentation of environment-sensitive parts of the metabolic network within glycosomes would, through this way of organelle renewal, enable the parasites to adapt rapidly and efficiently to the new conditions.