Zingiber officinale extract exhibits antidiabetic potential via modulating glucose uptake, protein glycation and inhibiting adipocyte differentiation: an in vitro study

• Published in: Journal of the science of food and agriculture Vol. 92; no. 9; pp. 1948 - 1955
• Main Authors: Rani, M Priya, Krishna, Mahesh S, Padmakumari, Keezheveettil P, Raghu, K Gopal, Sundaresan, Andikannu
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-07-2012; Wiley; John Wiley and Sons, Limited
• Subjects: adipocyte differentiation; Adipocytes - cytology; Adipocytes - drug effects; Adipogenesis - drug effects; Animals; Antioxidants; Antioxidants - pharmacology; Antioxidants - therapeutic use; Biological and medical sciences; Biological Transport - drug effects; Biphenyl Compounds - metabolism; Catechols - pharmacology; Catechols - therapeutic use; Cell Membrane - drug effects; Cell Membrane - metabolism; Cells; Diabetes Mellitus - drug therapy; Fatty Alcohols - pharmacology; Fatty Alcohols - therapeutic use; flow cytometry; Flowers & plants; Food industries; Food science; Fundamental and applied biological sciences. Psychology; Gene expression; Glucose; Glucose - metabolism; Glucose Transporter Type 4 - metabolism; Glut 4; Glycation End Products, Advanced - antagonists & inhibitors; HPLC; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; L6 mouse myoblasts; Mice; Muscle Fibers, Skeletal - drug effects; Muscle Fibers, Skeletal - metabolism; Myoblasts - drug effects; Myoblasts - metabolism; Phytotherapy; Picrates - metabolism; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Proteins - metabolism; Rhizome; T cell receptors; Zingiber officinale - chemistry; Adipocyte; Flow cytometry; Monocotyledones; L6 mouse myoblasts; HPLC chromatography; Extract; Glucose; Glycation; Hypoglycemic agent; Angiospermae; Carbohydrate; Zingiber officinale; HPLC; adipocyte differentiation; Rodentia; Glut 4; In vitro; Uptake; Protein; Vertebrata; Mammalia; Mouse; Spermatophyta; Differentiation; Zingiberaceae
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.5567

BACKGROUND: Ginger, the rhizome of Zingiber officinale Roscoe (Zingiberaceae), a perennial herbaceous plant is native to Southern Asia. Study was aimed to evaluate antioxidant and antidiabetic potential of ginger extract and its characterization. Possible mode of action to elicit antidiabetic activity was also evaluated.
METHODS AND RESULTS: Ethyl acetate extract of ginger (EAG) was evaluated for its antioxidant activity in terms of DPPH radical scavenging potential with an IC50 value of 4.59 µg/ml. Antidiabetic activity of EAG was evaluated by estimating antiglycation potential (IC50 290.84 µg/ml). HPLC profiling of EAG revealed the presence of phenolic components, gingerol and shoagol as major constituents. After determining sub‐toxic concentration of EAG (50 µg/ml), efficacy of extract to enhance glucose uptake in cell lines were checked in L6 mouse myoblast and myotubes. EAG was effective at 5 µg/ml concentration in both cases. Antibody based studies in treated cells revealed the effect of EAG in expressing Glut 4 in cell surface membrane compared to control.
CONCLUSION: The antidiabetic effect of ginger was experimentally proved in the study and has concluded that the activity is initiated by antioxidant, antiglycation and potential to express or transport Glut4 receptors from internal vesicles. Copyright © 2012 Society of Chemical Industry