Mediation of oxidative stress in hypothalamic ghrelin‐associated appetite control in rats treated with phenylpropanolamine

• Published in: Genes, brain and behavior Vol. 16; no. 4; pp. 439 - 448
• Main Authors: Yu, C.‐H., Chu, S.‐C., Chen, P.‐N., Hsieh, Y.‐S., Kuo, D.‐Y.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-04-2017; John Wiley & Sons, Inc
• Subjects: Animals; Anorexia - chemically induced; Antioxidants; Appetite - drug effects; Appetite - physiology; Body Weight; Brain; CART; Dietary fiber; Eating - drug effects; Feeding Behavior - drug effects; ghrelin; Ghrelin - metabolism; Hypothalamus - drug effects; Hypothalamus - metabolism; Male; Neuropeptide Y - metabolism; NPY; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - physiology; Peptide Hormones - pharmacology; phenylpropanolamine; Phenylpropanolamine - pharmacology; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Receptors, Ghrelin - antagonists & inhibitors; Receptors, Ghrelin - metabolism; Rodents; Brain; ghrelin; phenylpropanolamine; NPY; oxidative stress; CART
• ISSN: 1601-1848; 1601-183X
• DOI: 10.1111/gbb.12360

Phenylpropanolamine (PPA)‐induced appetite control is associated with oxidative stress in the hypothalamus. This study explored whether hypothalamic antioxidants participated in hypothalamic ghrelin system‐associated appetite control in PPA‐treated rats. Rats were given PPA daily for 4 days, and changes in food intake and the expression of neuropeptide Y (NPY), the cocaine‐ and amphetamine‐regulated transcript (CART), superoxide dismutase, catalase, ghrelin, acyl ghrelin (AG), ghrelin O‐acyltransferase (GOAT) and the ghrelin receptor (GHSR1a) were examined and compared. Results showed that both food intake and the expression of NPY and ghrelin/AG/GOAT/GHSR1a decreased in response to PPA treatment with maximum decrease on Day 2 of the treatment. In contrast, the expression of antioxidants and CART increased, with the maximum increase on Day 2, with the expression opposite to that of NPY and ghrelin. A cerebral infusion of either a GHSR1a antagonist or reactive oxygen species scavenger modulated feeding behavior and NPY, CART, antioxidants and ghrelin system expression, showing the involvement of ghrelin signaling and oxidative stress in regulating PPA‐mediated appetite control. We suggest that hypothalamic ghrelin signaling system, with the help of antioxidants, may participate in NPY/CART‐mediated appetite control in PPA‐treated rats.
We suggest that hypothalamic ghrelin signaling system, with the help of antioxidants, may participate in neuropeptide Y/cocaine‐ and amphetamine‐regulated transcript‐mediated appetite control in phenylpropanolamine‐treated rats.