Immunoaffinity extraction and tandem mass spectrometric analysis of human chorionic gonadotropin in doping analysis

A confirmatory and quantitative HPLC–tandem mass spectrometry (MS–MS) method for human chorionic gonadotropin hormone (hCG) at concentrations as low as 5 IU/l following immunoaffinity extraction of the glycoprotein from urine was developed. The extraction method involved retention of urinary hCG in...

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Bibliographic Details
Published in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 792; no. 2; pp. 187 - 196
Main Authors:	Gam, Lay-Harn, Tham, Sock-Ying, Latiff, Aishah
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 25-07-2003 Elsevier Science
Subjects:	Amino Acid Sequence Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Chorionic gonadotropin Chorionic Gonadotropin - chemistry Chorionic Gonadotropin - urine Chromatography, Affinity - methods Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology Glycoproteins Humans Molecular Sequence Data Peptide Mapping Proteins Spectrometry, Mass, Electrospray Ionization - methods Glycoproteins Chorionic gonadotropin Human Urine Biological fluid Antibody Doping Glycoprotein HPLC chromatography Urinary retention Antigen Protein hormone Analysis method Affinity chromatography Mass spectrometry MS/MS Chorionic gonadotrophin Extraction Quantitative analysis
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