Determination of levofloxacin in plasma, bronchoalveolar lavage and bone tissues by high-performance liquid chromatography with ultraviolet detection using a fully automated extraction method

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 799; no. 1; pp. 165 - 172
• Main Authors: Djabarouti, S., Boselli, E., Allaouchiche, B., Ba, B., Nguyen, A.T., Gordien, J.B., Bernadou, J.M., Saux, M.C., Breilh, D.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05-01-2004; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Anti-Infective Agents - analysis; Anti-Infective Agents - blood; Anti-Infective Agents - pharmacokinetics; Automation; Biological and medical sciences; Bone and Bones - chemistry; Bronchoalveolar Lavage Fluid - chemistry; Calibration; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Levofloxacin; Medical sciences; Ofloxacin - analysis; Ofloxacin - blood; Ofloxacin - pharmacokinetics; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Levofloxacin; Biological fluid; UVA radiation; HPLC chromatography; Blood plasma; Osteoarticular system; Fluoroquinolone derivatives; Ultraviolet detector; Bronchoalveolar lavage; Bone; Antibacterial agent; Quinolone derivatives; Quantitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2003.10.031

The aim of this study was to develop a specific and sensitive high-performance liquid chromatographic (HPLC) assay for the determination of levofloxacin in human plasma, bronchoalveolar lavage and bone tissues. The sample extraction was based on a fully automated liquid–solid extraction with an OASIS cartridge. The method used ultraviolet detection set at a wavelength of 299
nm and a separation with a Supelcosil ABZ+ column. The assay has been found linear over the concentration range 0.25–25
μg/ml for levofloxacin in plasma, 1–6
μg/ml in bronchoalveolar lavage and 0.5–10
μg/g for bone tissues and it provided good validation data for accuracy and precision. The assay will be applied to determine the penetration of levofloxacin in human bronchoalveolar lavage (BAL) and bone tissues during pharmacokinetic steady state.