Classification of Arcobacter species isolated from aborted pig fetuses and sows with reproductive problems in Brazil

• Published in: Veterinary microbiology Vol. 57; no. 4; pp. 347 - 354
• Main Authors: de Oliveira, S.J., Baetz, A.L., Wesley, I.V., Harmon, K.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 16-10-1997; Elsevier Science
• Subjects: Abortion, Veterinary - microbiology; Animal bacterial diseases; Animals; Arcobacter butzleri; Arcobacter cryaerophilus; Bacterial diseases; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Brazil; Fallopian Tubes - microbiology; Female; Fetus - microbiology; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - drug effects; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacterial Infections - classification; Gram-Negative Bacterial Infections - microbiology; Gram-Negative Bacterial Infections - veterinary; Infectious diseases; Infertility, Female - microbiology; Infertility, Female - veterinary; Kidney - embryology; Kidney - microbiology; Liver - embryology; Liver - microbiology; Medical sciences; Microbial Sensitivity Tests; Microbiology; Nalidixic Acid - pharmacology; PCR; Placenta - microbiology; Porcine abortion; Pregnancy; Restriction fragment length polymorphism; Swine; Swine Diseases; Uterus - microbiology; South America; Brazil; America; Arcobacter cryaerophilus; Restriction fragment length polymorphism; Porcine abortion; Arcobacter butzleri; PCR; Reproduction diseases; Identification; Species specificity; Female genital diseases; Pig; Infection; Polymerase chain reaction; Fetal diseases; Vertebrata; Mammalia; Animal; DNA; Bacteriosis; Bacteria; Female; Artiodactyla; Diagnosis; Veterinary; Detection; Ungulata
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/S0378-1135(97)00106-5

Seventeen field isolates of
Arcobacter species were recovered in Brazil from aborted porcine fetal livers (
n = 3), kidneys (
n = 2), and thoracic fluid (
n = 1).
Arcobacter species were also recovered from uterine and oviductal tissues (
n = 5) and a placenta from sows with reproductive problems. These isolates were initially presumed to be
Arcobacter cryaerophilus on the basis of aerobic growth at 30°C, indoxyl acetate hydrolysis, catalase and oxidase reactions, growth on MacConkey agar, sensitivity to 3.5% sodium chloride, and susceptibility to nalidixic acid (40 mg/ml). The isolates were confirmed as
Arcobacter using polymerase chain reaction, and were classified as
A. cryaerophilus 1A (24%),
A. cryaerophilus 1B (71%), and
A. butzleri (6%) using restriction fragment length polymorphism.