β‐Taxilin participates in differentiation of C2C12 myoblasts into myotubes

• Published in: Experimental cell research Vol. 345; no. 2; pp. 230 - 238
• Main Authors: Sakane, Hiroshi, Makiyama, Tomohiko, Nogami, Satoru, Horii, Yukimi, Akasaki, Kenji, Shirataki, Hiromichi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15-07-2016
• Subjects: 60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; Animals; C2C12; Cell Differentiation; Cercopithecus aethiops; COS Cells; Dysbindin; Dystrophin-Associated Proteins - metabolism; FLUORESCENCE; FUNCTIONS; Gene Knockdown Techniques; GENES; Green Fluorescent Proteins - metabolism; HEART; Humans; Immunoprecipitation; MEMBRANES; Mice; Muscle Fibers, Skeletal - cytology; Muscle Fibers, Skeletal - metabolism; MYOBLASTS; Myoblasts - cytology; Myoblasts - metabolism; Myogenesis; MYOSIN; Nuclear Proteins - metabolism; OXIDOREDUCTASES; PHOSPHATES; PLANT TISSUES; Protein Binding; Proto-Oncogene Proteins c-myc - metabolism; RECEPTORS; RNA; SORTING; Taxilin; TRANSCRIPTION; TRANSFERRIN; Vesicular Transport Proteins; RT; Vamp; MHC; DM; siRNA; Taxilin; Myogenesis; GFP; TfnR; DMEM; C2C12; SNX; GAPDH; Dysbindin
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2016.05.016

Myogenesis is required for the development of skeletal muscle. Accumulating evidence indicates that the expression of several genes are upregulated during myogenesis and these genes play pivotal roles in myogenesis. However, the molecular mechanism underlying myogenesis is not fully understood. In this study, we found that β-taxilin, which is specifically expressed in the skeletal muscle and heart tissues, was progressively expressed during differentiation of C2C12 myoblasts into myotubes, prompting us to investigate the role of β-taxilin in myogenesis. In C2C12 cells, knockdown of β-taxilin impaired the fusion of myoblasts into myotubes, and decreased the diameter of myotubes. We also found that β-taxilin interacted with dysbindin, a coiled-coil-containing protein. Knockdown of dysbindin conversely promoted the fusion of myoblasts into myotubes and increased the diameter of myotubes in C2C12 cells. Furthermore, knockdown of dysbindin attenuated the inhibitory effect of β-taxilin depletion on myotube formation of C2C12 cells. These results demonstrate that β-taxilin participates in myogenesis through suppressing the function of dysbindin to inhibit the differentiation of C2C12 myoblasts into myotubes.
•β‐Taxilin is progressively expressed during differentiation of C2C12 cell.•Knockdown of β-taxilin impaired C2C12 myotube formation.•β‐Taxilin interacted with dysbindin.•Knockdown of dysbindin promoted C2C12 myotube formation.•The function of β-taxilin in C2C12 myotube formation depends on dysbindin.