Simultaneous degradation of phytic acid and starch by an industrial strain of Saccharomyces cerevisiae producing phytase and α-amylase

• Published in: Biotechnology letters Vol. 30; no. 12; pp. 2125 - 2130
• Main Authors: Lim, Mi-Hyeon, Lee, Ok-Hee, Chin, Jong-Eon, Ko, Hyun-Mi, Kim, Il-Chul, Lee, Hwanghee Blaise, Im, Suhn-Young, Bai, Suk
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01-12-2008; Springer Netherlands; Springer
• Subjects: 6-Phytase - genetics; 6-Phytase - metabolism; alpha-amylase; alpha-Amylases - genetics; alpha-Amylases - metabolism; Animal Feed; Animals; Applied Microbiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; DNA; feeds; foods; Fundamental and applied biological sciences. Psychology; gene expression; genes; Genes, Fungal; Humans; Hydrolysis; Life Sciences; Microbiology; myo-inositol; Original Research Paper; phytases; phytic acid; Phytic Acid - metabolism; Polymerase Chain Reaction; Recombinant Proteins - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Seeds; sodium; starch; Starch - metabolism; Transformation, Genetic; yeasts; Industrial yeast; Phytase; Yeast; α-Amylase; Simultaneous degradation; Ascomycota; 4-Phytase; Enzyme; Starch; Phosphoric monoester hydrolases; Esterases; Fungi; Glycosylases; Degradation; Simultaneous degradation, Yeast; α-Amylase, Industrial yeast; Glycosidases; Hydrolases; Saccharomyces cerevisiae
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-008-9799-x

Phytase liberates inorganic phosphate from phytic acid (myo-inositol hexakisphosphate) which is the major phosphate reserve in plant-derived foods and feeds. An industrial strain of Saccharomyces cerevisiae expressing the Debaryomyces castellii phytase gene (phytDc) and D. occidentalis α-amylase gene (AMY) was developed. The phytDc and AMY genes were constitutively expressed under the ADC1 promoter in S. cerevisiae by using the δ-integration system, which contains DNA derived exclusively from yeast. The recombinant industrial strain secreted both phytase and α-amylase for the efficient degradation of phytic acid and starch as main components of plant seeds. This new strain hydrolyzed 90% of 0.5% (w/v) sodium phytate within 5 days of growth and utilized 100% of 2% (w/v) starch within 48 h simultaneously.