Acquired AmpC β-Lactamases among Enterobacteriaceae from Healthy Humans and Animals, Food, Aquatic and Trout Aquaculture Environments in Portugal

• Published in: Pathogens (Basel) Vol. 9; no. 4; p. 273
• Main Authors: Gonçalves Ribeiro, Teresa, Novais, Ângela, Machado, Elisabete, Peixe, Luísa
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09-04-2020; MDPI
• Subjects: Animals; Antibiotics; Aquaculture; Aquatic animals; Aquatic environment; Carcasses; Cephalosporins; Chickens; Cloning; CMY-2; Drug resistance; E coli; Enterobacteriaceae; Epidemiology; Escherichia coli; Food; Health care; Hybridization; Investigations; Letter; Livestock; Origins; Phylogenetics; Plasmids; Poultry; Public health; Salmonella; ST48; ST665; Swine; Portugal; CMY-2; ST48; plasmids; ST665; Escherichia coli
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens9040273

We aimed to investigate the occurrence of acquired AmpC β-lactamases (qAmpC), and characterize qAmpC-producing
from different non-clinical environments in Portugal. We analysed 880
resistant to third-generation cephalosporins recovered from 632 non-clinical samples [healthy human and healthy animal (swine, chickens) faeces; uncooked chicken carcasses; aquatic and trout aquaculture samples]. Bacterial and qAmpC identification, antibiotic susceptibility, clonal (PFGE, MLST) and plasmid (S1-/I-
I-PFGE, replicon typing, hybridization) analysis were performed using standard methods. The occurrence of qAmpC among
from non-clinical origins was low (0.6%; n = 4/628 samples), corresponding to CMY-2-producing
from three healthy humans (HH) and one uncooked chicken carcass (UCC). We highlight a slight increase in CMY-2 human faecal carriage in the two periods sampled [1.0% in 2013-2014 versus 0% in 2001-2004], which is in accordance with the trend observed in other European countries. CMY-2-producing
belonged to B2
-ST4953 (n = 2, HH), A
-ST665 (n = 1, HH) or A
-ST48 (n = 1, UCC) clones.
was identified in non-typeable and IncA/C
plasmids. This study is one of the few providing an integrated evaluation of the qAmpC-producing
occurrence, which was low, from a very large collection of different non-clinical origins. Further surveillance in contemporary collections can provide an integrated epidemiological information of potential shifts in reservoirs, transmission routes and mechanisms of dissemination of
in non-clinical settings.