Bioprotective potential of lactic acid bacteria for Salmonella biocontrol in vitro

• Published in: Veterinary research communications Vol. 47; no. 3; pp. 1357 - 1368
• Main Authors: Evangelista, Alberto Gonçalves, Matté, Eduardo Henrique Custódio, Corrêa, Jessica Audrey Feijó, Gonçalves, Francieli Dalvana Ribeiro, dos Santos, João Vitor Garcia, Biauki, Gabrieli Camila, Milek, Mônica Moura, Costa, Leandro Batista, Luciano, Fernando Bittencourt
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-09-2023; Springer Nature B.V
• Subjects: Bacteria; Biological control; Biomedical and Life Sciences; Dietary supplements; Encapsulation; Lactic acid bacteria; Life Sciences; Meat products; Probiotics; Salmonella; Veterinary Medicine/Veterinary Science; Zoology; Swine production; Animal production; Bacterial encapsulation; Bioprotective bacteria; Chicken production
• DOI: 10.1007/s11259-023-10083-4
• EISSN: 1573-7446
• ISSN: 0165-7380

Lactic acid bacteria (LAB) are an important option for
Salmonella
control in animal production, resulting in lower antibiotic use. The objective of this research was to isolate LAB from meat products and from commercial probiotics sold as nutritional supplements for
in vitro
verification of their bioprotective potential. Eleven bacteria were identified as
Pediococcus acidilactici
, two as
Lacticaseibacillus rhamnosus
, one as
Lacticaseibacillus paracasei paracasei
, one as
Limosilactobacillus fermentum
, and one as a consortium of
Lactobacillus delbrueckii bulgaricus
and
L. fermentum
. All bacteria showed inhibitory activity against
Salmonella
, with emphasis on the inhibition of
P. acidilactici
PUCPR 011 against
Salmonella
Enteritidis 33SUSUP,
S.
Enteritidis 9SUSP,
S.
Enteritidis 56301,
S.
Enteritidis CRIFS 1016,
Salmonella
Typhimurium ATCC
™
14,028
®
, and
Salmonella
Gallinarum AL 1138, with inhibition halos of 7.3 ± 0.5 mm, 7.7 ± 1.0 mm, 9.0 ± 1.8 mm, 7.3 ± 0.5 mm, 7.7 ± 1.0 mm, and 7.3 ± 0.5, respectively. The isolates
P. acidilactici
PUCPR 011,
P. acidilactici
PUCPR 012,
P. acidilactici
PUCPR 014,
L. fermentum
PUCPR 005,
L. paracasei paracasei
PUCPR 013, and
L. rhamnosus
PUCPR 010 showed inhibition greater than 2 mm against at least 3
Salmonella
and were used for encapsulation and
in vitro
digestion. The encapsulation efficiency ranged from 76.89 ± 1.54 to 116.48 ± 2.23%, and the population after 12 months of storage was from 5.31 ± 0.17 to 9.46 ± 0.09 log CFU/g. When simulating swine and chicken digestion, there was a large reduction in bacterial viability, stabilizing at concentrations close to 2.5 log CFU/mL after the analyses. The analyzed bacteria showed strong
in vitro
bioprotective potential; further analyses are required to determine
in vivo
effectiveness.