Spontaneous and Microbiota-Driven Degradation of Anthocyanins in an In Vitro Human Colon Model

• Published in: Molecular nutrition & food research Vol. 67; no. 19; p. e2300036
• Main Authors: Shehata, Emad, Day-Walsh, Priscilla, Kellingray, Lee, Narbad, Arjan, Kroon, Paul A
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-10-2023; John Wiley and Sons Inc
• Subjects: Anthocyanins; Anthocyanins - metabolism; Anthocyanins - pharmacology; Bioavailability; Colon; Colon - metabolism; Degradation; Diet; Humans; Intestinal microflora; Liquid chromatography; Metabolism; Metabolites; Microbiota; Microorganisms; Phenolic compounds; Phenols; Phenols - metabolism; anaerobic fermentation; polyphenols; microbial metabolism; bioavailability; flavonoids; colon model; gut microbiota
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.202300036

The consumption of dietary anthocyanins is associated with various health benefits. However, anthocyanins are poorly bioavailable, and most ingested anthocyanins will enter the colon where they are degraded to small phenolic metabolites that are the main absorbed forms. Little is known about the processes of anthocyanin degradation in the gut and the role of the human gut microbiota. This study aims to determine the contribution of spontaneous and microbiota-dependent degradation of anthocyanins in the human colon.
Purified anthocyanin extracts from black rice and bilberry were incubated in an in vitro human fecal-inoculated pH-controlled colon model over 24 h and anthocyanins were analyzed using HPLC-DAD. The study shows that the loss of anthocyanins occurs both spontaneously and as a consequence of metabolism by the gut microbiota. The study observes that there is high variability in spontaneous degradation but only modest variation in total degradation, which included the microbiota-dependent component. The degradation rate of anthocyanins is also shown to be dependent on the B-ring substitution pattern and the type of sugar moiety, both for spontaneous and microbiota-dependent degradation.
Anthocyanins are completely degraded in a model of the human colon by a combination of spontaneous and microbiota-dependent processes.