Screen-printed electrodes modified with carbon black and polyelectrolyte films for determination of cancer marker carbohydrate antigen 19-9

• Published in: Mikrochimica acta (1966) Vol. 187; no. 7; p. 417
• Main Authors: Ibáñez-Redín, Gisela, Materon, Elsa M., Furuta, Roberto H. M., Wilson, Deivy, do Nascimento, Gustavo Freitas, Melendez, Matias E., Carvalho, André L., Reis, Rui Manuel, Oliveira, Osvaldo N., Gonçalves, Débora
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-07-2020; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Antibodies; Antigens; Biomarkers; Cancer; Carbohydrates; Carbon; Carbon black; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Coated electrodes; Electric properties; Electrodes; Immunosensors; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Pancreatic cancer; Polyelectrolytes; Cancer biomarkers; CA 19-9; Polyelectrolytes; Layer-by-layer films; Carbon black; Screen-printed electrodes
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-020-04404-6

Electrochemical immunosensors have been developed to determine the carbohydrate antigen 19-9 (CA19-9). They are based on screen-printed carbon electrodes (SPCEs) coated with layer-by-layer (LbL) films of carbon black (CB) and polyelectrolytes. Owing to a suitable choice of LbL film architecture, the procedures for immobilization of anti-CA19-9 antibodies on the electrode surfaces were straightforward. Mechanically flexible immunosensors were capable of detecting CA19-9 within a dynamic range of 0.01 to 40 U mL
−1
and a limit of detection of 0.07 U mL
−1
using differential pulse voltammetry. In addition to detecting CA19-9 at clinically relevant concentrations for pancreatic cancer in standard solutions, the immunosensors provide the determination of CA19-9 on cell lysate and human serum samples. Using LbL films led to immunosensors with superior performance compared to similar systems obtained by drop casting. The fabrication of this relatively simple, inexpensive platform is a demonstration that SPCEs modified with cost-effective materials are able to detect cancer biomarkers and may be adapted to other disposable immunosensors.
Graphical abstract
Schematic representation of assembly and characterization of electrochemical immunosensors for the determination of carbohydrate antigen 19-9 based on printed electrodes modified with composites of carbon black and polyelectrolyte films