A Sea Urchin Gene Encoding Dystrophin-Related Proteins

• Published in: Human molecular genetics Vol. 7; no. 4; pp. 581 - 588
• Main Authors: Wang, Jun, Pansky, Avi, Venuti, Judith M., Yaffe, David, Nudel, Uri
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-04-1998
• Subjects: Amino Acid Sequence; Animals; Biological and medical sciences; Cytoskeletal Proteins - genetics; DNA, Complementary - isolation & purification; Drosophila; Dystrophin - analogs & derivatives; Dystrophin - genetics; Dystrophin - metabolism; Exons - genetics; Fundamental and applied biological sciences. Psychology; Genes. Genome; Humans; Introns - genetics; Marine; Membrane Proteins - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Muscle Proteins; Phylogeny; Polymerase Chain Reaction; Sea Urchins - genetics; Sequence Homology, Amino Acid; Strongylocentrotus purpuratus; Torpedo; Transcription, Genetic; Utrophin; Nucleotide sequence; Echinodermata; Homology; Gene organization; Protein; Strongylocentrotus purpuratus; Molecular evolution; Gene; Domain structure; Duchenne muscular dystrophy; Genetics; Echinoidea; Invertebrata; Dystrophin
• ISSN: 0964-6906; 1460-2083; 1460-2083
• DOI: 10.1093/hmg/7.4.581

The gene which is defective in Duchenne muscular dystrophy (DMD) is the largest known gene. The product of the gene in muscle, dystrophin, is a 427 kDa protein. The same gene encodes at least six additional products: two non-muscle dystrophin isoforms transcribed from promoters located in the 5′-end region of the gene and four smaller proteins transcribed from internal promoters located further downstream. Several other genes, encoding evolutionarily related proteins, have been identified. These include a structurally very similar gene in vertebrates encoding utrophin (DRP1), which is closely related to dystrophin, and a number of small and simple genes in vertebrates or invertebrates encoding proteins similar to some of the small products of the DMD gene. We have isolated a sea urchin gene showing very strong sequence and structural homology with the DMD and utrophin genes. Sequence and intron/exon structure similarities suggest that this gene is related to a precursor of both the DMD gene and the gene encoding utrophin. The sea urchin gene has the unique complex structure of the DMD gene. There is at least one, and possibly more, product(s) transcribed from internal promoters, as well as a large product of >300 kDa containing at least three of the four major domains of dystrophin. The small product seems to be evolutionarily related to Dp116, one of the small products of the human DMD gene. Partial characterization of this gene helped us to construct an evolutionary tree connecting the vertebrate dystrophin gene family with related genes in invertebrates. The constructed evolutionary tree also implies that the vertebrate small and simple structured gene encoding a Dp71-like protein, called DRP2, evolved from the dystrophin/utrophin ancestral large and complex gene by a duplication of only a small part of the gene.