Relationship between macrophage differentiation and the chemotactic activity toward damaged myoblast cells

• Published in: Journal of immunological methods Vol. 393; no. 1-2; pp. 61 - 69
• Main Authors: Uchida, Masataka, Oyanagi, Eri, Miyachi, Motohiko, Yamauchi, Akira, Yano, Hiromi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31-07-2013
• Subjects: Actins - immunology; Actins - metabolism; Animals; CCR2; Cell Differentiation - immunology; Cell Line; Chemokine CCL2 - immunology; Chemokine CCL2 - metabolism; Chemotaxis - immunology; Dexamethasone; F-actin; Lipopolysaccharide; Macrophages - immunology; Macrophages - metabolism; Macrophages - pathology; Mice; Muscle, Skeletal - immunology; Muscle, Skeletal - metabolism; Muscle, Skeletal - pathology; Muscular Diseases - immunology; Muscular Diseases - metabolism; Muscular Diseases - pathology; Myoblasts - immunology; Myoblasts - metabolism; Myoblasts - pathology; Receptors, CCR2 - immunology; Receptors, CCR2 - metabolism; TAXIScan; Lipopolysaccharide; Dexamethasone; F-actin; TAXIScan; CCR2
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2013.04.007

We investigated the effect of macrophage differentiation on the chemotactic activity to invade local damaged myoblasts using in vitro models of muscle injury. We found that: 1) the chemotactic activity of macrophages toward areas of damaged myoblasts might be induced more by live myoblasts than dead ones, 2) the chemotactic activity of macrophages is not due to velocity, but depends on the directionality toward damaged myoblast cells, and 3) macrophage differentiation strongly influence the chemotactic activity toward damaged myoblast cells through the expression of CCR2 and/or F-actin.
•Macrophage chemotaxis is induced more by live myoblasts than dead ones.•Macrophage chemotaxis depends on the directionality toward damaged myoblast cells.•Macrophage differentiation influences the chemotaxis toward damaged myoblast cells.