Development of real‐time RT‐PCR assays for detection of three classes of HHV‐6A gene transcripts

• Published in: Journal of medical virology Vol. 89; no. 10; pp. 1830 - 1836
• Main Authors: Ihira, Masaru, Urashima, Akiko, Miura, Hiroki, Hattori, Fumihiko, Kawamura, Yoshiki, Sugata, Ken, Yoshikawa, Tetsushi
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-10-2017
• Subjects: Activation; Amplification; Assaying; Coefficient of variation; DNA, Viral - genetics; Female; Genetics; Herpes viruses; Herpesvirus 6, Human - classification; Herpesvirus 6, Human - genetics; Herpesvirus 6, Human - isolation & purification; HHV‐6A; HSCT; Humans; Latent infection; Leukocytes (mononuclear); Leukocytes, Mononuclear - virology; Male; Patients; Peripheral blood mononuclear cells; Plasmids; Polymerase chain reaction; Real time; Real-Time Polymerase Chain Reaction - methods; real‐time PCR; Reliability analysis; Reproducibility of Results; Reverse transcription; Roseolovirus Infections - diagnosis; Roseolovirus Infections - virology; Sensitivity and Specificity; Severe combined immunodeficiency; Severe Combined Immunodeficiency - virology; variant‐specific; Viral infections; Viral Load; Viremia; Virology; Virus Latency; HHV-6A; variant-specific; real-time PCR; HSCT
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.24862

Human herpesvirus 6 (HHV‐6), a member of the betaherpesvirus family, has two distinct species: HHV‐6A and HHV‐6B. HHV‐6B real‐time reverse transcription polymerase chain reaction (RT‐PCR) has been used to distinguish between active and latent viral infection. In this study, we developed a real‐time RT‐PCR assay to detect HHV‐6A‐specific transcripts and evaluated its reliability for analysis of clinical samples. To develop HHV‐6A‐specific real‐time RT‐PCR assays, three different classes of gene transcripts (immediate early: U90; early: U12; and late: U100) were selected as targets. Serial d ilutions of plasmid DNAs containing target sequences and RNAs extracted from HHV‐6A‐infected cells were used to determine assay specificity and sensitivity. Peripheral blood mononuclear cells (PBMCs) collected from patients with either primary or reactivated HHV‐6B infection, and one patient with X‐linked severe combined immunodeficiency (X‐SCID) with HHV‐6A reactivation, were used to evaluate assay reliability. The HHV‐6A‐specific real‐time RT‐PCR assays amplified plasmids containing the target sequences at concentrations between 10 and 1 × 106 copies per reaction. The intra‐assay coefficients of variation were less than 5%. The three classes of HHV‐6A gene transcripts were not detected in any HHV‐6B sample isolated from the patients. In the X‐SCID patient, high copy numbers of HHV‐6A U12 and U100 transcripts were detected in PBMC samples during viremia. Thus, we successfully established highly sensitive and reproducible real‐time RT‐PCR methods targeting three classes of HHV‐6A gene transcripts. This method should be useful for discriminating active HHV‐6A infection from either latent infection or chromosomally integrated HHV‐6A (ciHHV‐6A).