Paper mulberry leaves as a potential sterilant: evidence from Microtus fortis -a laboratory study

• Published in: Frontiers in plant science Vol. 14; p. 1092792
• Main Authors: Wang, Shuangye, Chen, Junzhi, Zhao, Yunlin, Zhang, Meiwen, Zhang, Chen, He, Jianing, Wei, Lichuan, Xu, Zhenggang
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 09-06-2023
• Subjects: Broussonetia papyrifera; Microtus fortis; organ coefficient; Plant Science; reproduction; rodenticide; sex hormone; sex hormone; organ coefficient; Broussonetia papyrifera; rodenticide; Microtus fortis; reproduction
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2023.1092792

The Yangtze vole (
) is a small herbivorous rodent that usually causes damage to crops and forests in China. Various measures were used to control their population including chemical rodenticides. However, rodenticides may cause secondary damage to the environment and the ecosystem. Therefore, the development of new rodent sterilants is urgent. Considering that some compounds of paper mulberry leaves have been verified that can inhibit the biosynthesis of sexual hormone, we aimed to explore the antifertility effect of paper mulberry leaves on
.
In this study, voles were divided into three groups including a male group, a female group, and a breeding group, and paper mulberry leaves were added into basal fodder of voles maintained in laboratory, of which the proportion of leaf weight was 50%. In each group, voles were fed with mixed fodder as treatment (BP) and voles were fed with basal fodder as contrast (CK).
After feeding for more than 1 month, the results indicated that paper mulberry leaves attracted voles to feed, but inhibited their growth and reproduction. Since the second week, food intakes of BP have been significantly higher than CK (
< 0.05). However, weights of voles in male and female groups were 72.283 ± 7.394 g and 49.717 ± 2.278 g in the fifth week, and both were significantly reduced compared with their original weight (
< 0.05). Meanwhile, testicular volumes of male voles fed with BP were significantly smaller than CK (former: 318.000 ± 44.654 mm
, latter: 459.339 ± 108.755 mm
); the testosterone level, sperm number, and vitality of BP were obviously weaker than CK. Female uteruses and oophoron of BP grew slower, and the organ coefficients of uterus and oophoron fed BP were both significantly lower than CK (
< 0.05). The first reproduction of BP couple voles spent 45 days, while CK spent only 21 days. These results suggest that paper mulberry leaves could be the potential resource to produce sterilants to control rodent populations by delaying their sexual growth and reproduction. If it was practical, the apparent advantages of paper mulberry are that it is an abundant resource and the inhibitory effect could be effective in both male and female individuals. Our conclusion also supports the transformation of rodent management from lethal management to fertility control, which would be more ecologically friendly to agriculture and the ecosystem.