Oxidized low-density lipoproteins accumulate in rat lung after experimental lung edema induced by alpha- naphthylthiourea (ANTU)

• Published in: Pharmacological research Vol. 50; no. 6; pp. 585 - 591
• Main Authors: Sipahi, Emine Yilmaz, Ozel Tekin, Ishak, Comert, Mustafa, Barut, Figen, Ustun, Huseyin, Sipahi, Tunc Hakan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01-12-2004
• Subjects: Alpha-naphthylthiourea; Animals; Female; Lipoproteins, LDL - analysis; Lipoproteins, LDL - metabolism; Lung - chemistry; Lung - metabolism; Lung - pathology; Lung edema; Male; Oxidized low-density lipoprotein; Pulmonary Edema - chemically induced; Pulmonary Edema - metabolism; Pulmonary Edema - pathology; Rats; Thiourea - analogs & derivatives; Thiourea - toxicity; Alpha-naphthylthiourea; Oxidized low-density lipoprotein; Lung edema
• ISSN: 1043-6618; 1096-1186
• DOI: 10.1016/j.phrs.2004.04.003

Oxidation of the low-density lipoprotein (LDL) results in the production of modified LDLs. Oxidation of LDL cholesterol plays a role on the pathogenesis of endothelial dysfunction. This study was designed to investigate the possible participation of the oxidative modification of low density lipoprotein in the lung edema induced by alpha-naphthylthiourea (ANTU), which is a well-known noxious chemical agent on the lung endothelium. When ANTU injected intraperitoneally into rats (15
mg
kg
−1), it produced lung edema as indicated by an increase in lung weight/body weight (LW/BW) ratio and pleural effusion (PE) reaching a maximum within 4
h. A significant lung edema was observed 4
h after intraperitoneally injection of alpha-naphthylthiourea when compared with olive oil-injected control rats. On microscopic examination of alpha-naphthylthiourea-treated rats were shown to have severe lung injury, while no change was observed in olive oil-treated control rats. While there were no staining in control lungs, positive oxidized low-density lipoproteins immune-fluorescent staining were observed in lung edema group. Our study showed that oxidized low-density lipoprotein (oxLDL) accumulated in ANTU-induced lung damage. This is the first study in which accumulation of oxLDL molecules in the intact lung tissue were shown by fluorescent immune-staining method in experimental lung edema. The potential role of oxLDL in this pathology are still under investigation.