Uterine estrogen sulfatase activity. Influence of steroid hormones and adenine nucleotides

• Published in: Journal of steroid biochemistry Vol. 36; no. 4; p. 301
• Main Authors: Loza, M C, Valencia, A, Hicks, J J
• Format: Journal Article
• Language: English
• Published: England 1990
• Subjects: Adenine Nucleotides - pharmacology; Adenosine Diphosphate - pharmacology; Adenosine Triphosphate - pharmacology; Animals; Estradiol - pharmacology; Female; NAD - pharmacology; Ovariectomy; Progesterone - pharmacology; Rats; Sulfatases - antagonists & inhibitors; Sulfatases - metabolism; Uterus - enzymology
• ISSN: 0022-4731
• DOI: 10.1016/0022-4731(90)90221-D

Steroid sulfatase enzymes participate greatly in reproductive events. To date, estrogen sulfatase seems to have a regulatory role in the control of free estrogen levels in target tissues. The present study evaluates the participation of some adenine nucleotides in estrogen sulfatase kinetics. Using ADP, ATP, NAD and the combination of ADP + NAD or ATP + NAD it was found that adding either of the combined cofactors, the enzymatic activity increased more than 2.0 times. In ovariectomized rats, the corresponding mean enzyme activity was found to be higher than in intact rats. It was also found, in ovariectomized rats treated with ovarian hormones, an inhibition that was higher with estradiol-17 beta than with progesterone treatment. This data suggests that the estrogen sulfatase, being a hormone-dependent enzyme, participates in a new control mechanism of estrogen levels in presence of some cofactors and free steroids.