Suicide vectors for antibiotic marker exchange and rapid generation of multiple knockout mutants by allelic exchange in Gram-negative bacteria

Allelic exchange is frequently used in bacteria to generate knockout mutants in genes of interest, to carry out phenotypic analysis and learn about their function. Frequently, understanding of gene function in complex processes such as pathogenesis requires the generation of multiple mutant strains....

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Bibliographic Details
Published in:	Journal of microbiological methods Vol. 67; no. 3; pp. 395 - 407
Main Authors:	Ortiz-Martín, Inmaculada, Macho, Alberto P., Lambersten, Lotte, Ramos, Cayo, Beuzón, Carmen R.
Format:	Journal Article
Language:	English
Published:	Shannon Elsevier B.V 01-12-2006 Elsevier Science
Subjects:	Allelic exchange Anti-Bacterial Agents - pharmacology Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Chloramphenicol Resistance - genetics Drug Resistance, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genes, Regulator Genetic Engineering - methods Genetic Vectors Genetics, Microbial - methods Genic rearrangement. Recombination. Transposable element Gram-Negative Bacteria - genetics Kanamycin Resistance - genetics Microbiology Molecular and cellular biology Molecular genetics Mutagenesis, Insertional - methods Pathogenesis Plasmids Pseudomonas syringae Pseudomonas syringae - genetics Recombination, Genetic Selection, Genetic Type III secretion system Virulence Allelic exchange Type III secretion system Pseudomonas syringae Pathogenesis Virulence Pseudomonadales Multiple Secretion Marker Antibiotic Bacteria Pseudomonadaceae Conjugation Vector Gram negative bacteria
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