A simplified lysis method allowing the use of biotinylated probes in colony hybridization

A method is described for the lysis of bacterial cells grown on nitrocellulose filters which allows the use of nonradioactive (biotin labeled) probes in colony hybridization. Used in conjunction with a colorimetric assay involving streptavidin and alkaline phosphatase this lysis method allows the de...

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Bibliographic Details

Published in:	Analytical biochemistry Vol. 168; no. 2; p. 239
Main Authors:	Haas, M J, Fleming, D J
Format:	Journal Article
Language:	English
Published:	United States 01-02-1988
Subjects:	Bacteriolysis Biotin - pharmacology Clone Cells - analysis DNA, Bacterial Escherichia coli - genetics Hybridization, Genetic Plasmids
Online Access:	Get more information
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