Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs

• Published in: Biochemical and biophysical research communications Vol. 182; no. 3; p. 1416
• Main Authors: Owaki, H, Makar, R, Boulton, T G, Cobb, M H, Geppert, T D
• Format: Journal Article
• Language: English
• Published: United States 14-02-1992
• Subjects: Adult; Amino Acid Sequence; Animals; Base Sequence; Cell Line; DNA - genetics; DNA - isolation & purification; DNA, Neoplasm - genetics; Humans; Mice; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; Molecular Sequence Data; Monocytes - enzymology; Protein Kinases - genetics; Rats; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Homology, Nucleic Acid; T-Lymphocytes - enzymology
• ISSN: 0006-291X
• DOI: 10.1016/0006-291X(92)91891-S

Extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases. cDNAs for both human kinases were isolated and sequenced. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 1 were 88% and 96% identical, respectively, to the homologous rat sequences. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 2 were 90% and 98% identical, respectively, to the corresponding rat sequences. A human extracellular signal-regulated kinase 2 specific probe was used to demonstrate that the mRNA for this kinase was present in T cells and did not change with activation. The deduced protein sequences of both human kinases were greater than 95% identical to two Xenopus kinase sequences, indicating that these enzymes are highly conserved across species.