Phenocopies induced with antisense RNA identify the wingless gene

Earlier work suggested that the wingless gene of Drosophila is required for cooperation within discrete groups of cells during development. We show that antisense RNA made from a 3 kb transcript produces wingless mutant phenocopies when injected into wild-type eggs, proving that this transcript exec...

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Bibliographic Details
Published in:	Cell Vol. 50; no. 4; p. 659
Main Authors:	Cabrera, C V, Alonso, M C, Johnston, P, Phillips, R G, Lawrence, P A
Format:	Journal Article
Language:	English
Published:	United States 14-08-1987
Subjects:	Animals DNA - genetics Drosophila melanogaster - embryology Drosophila melanogaster - genetics Genes Insect Hormones - genetics Larva - ultrastructure Mice - genetics Morphogenesis Phenotype RNA - genetics RNA, Antisense Sequence Homology, Nucleic Acid
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Summary:	Earlier work suggested that the wingless gene of Drosophila is required for cooperation within discrete groups of cells during development. We show that antisense RNA made from a 3 kb transcript produces wingless mutant phenocopies when injected into wild-type eggs, proving that this transcript executes the wingless function. In the accompanying paper, Rijsewijk et al. report cloning of a Drosophila homolog (Dint-1) of the mouse int-1 gene and show that this gene is identical to wingless+. The partial sequence of the wingless cDNA that we have isolated is identical to part of the complete Dint-1 sequence described by Rijsewijk et al.
ISSN:	0092-8674
DOI:	10.1016/0092-8674(87)90039-0