The detection and enumeration of cytokine-secreting cells in mice and man and the clinical application of these assays

An in vitro assay for the detection and enumeration of mouse and human cytokine-secreting cells is described and some ways in which it may be used diagnostically are indicated. The assay is an extension of the ELISA plaque assay or ELISPOT assay and uses pairs of antibodies to capture and then visua...

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Bibliographic Details
Published in:	Journal of immunological methods Vol. 120; no. 1; p. 1
Main Authors:	Hutchings, P R, Cambridge, G, Tite, J P, Meager, T, Cooke, A
Format:	Journal Article
Language:	English
Published:	Netherlands 02-06-1989
Subjects:	Animals Antibodies, Monoclonal - immunology Biological Factors - metabolism Cells, Cultured Cytokines Enzyme-Linked Immunosorbent Assay - methods Humans Interferon-gamma - analysis Lymphokines - analysis Mice Muscles - cytology Tumor Necrosis Factor-alpha - analysis
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Summary:	An in vitro assay for the detection and enumeration of mouse and human cytokine-secreting cells is described and some ways in which it may be used diagnostically are indicated. The assay is an extension of the ELISA plaque assay or ELISPOT assay and uses pairs of antibodies to capture and then visually develop secreted lymphokines. In this way, it is possible to enumerate the specific cytokine-secreting cells. This assay may provide a valuable tool in the clinical investigation of the mechanisms of disease development and tissue destruction.
ISSN:	0022-1759
DOI:	10.1016/0022-1759(89)90281-0