BioBits™ Bright: A fluorescent synthetic biology education kit

Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow li...

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Published in:	Science advances Vol. 4; no. 8; p. eaat5107
Main Authors:	Stark, Jessica C, Huang, Ally, Nguyen, Peter Q, Dubner, Rachel S, Hsu, Karen J, Ferrante, Thomas C, Anderson, Mary, Kanapskyte, Ada, Mucha, Quinn, Packett, Jessica S, Patel, Palak, Patel, Richa, Qaq, Deema, Zondor, Tyler, Burke, Julie, Martinez, Thomas, Miller-Berry, Ashlee, Puppala, Aparna, Reichert, Kara, Schmid, Miriam, Brand, Lance, Hill, Lander R, Chellaswamy, Jemima F, Faheem, Nuhie, Fetherling, Suzanne, Gong, Elissa, Gonzalzles, Eddie Marie, Granito, Teresa, Koritsaris, Jenna, Nguyen, Binh, Ottman, Sujud, Palffy, Christina, Patel, Angela, Skweres, Sheila, Slaton, Adriane, Woods, TaRhonda, Donghia, Nina, Pardee, Keith, Collins, James J, Jewett, Michael C
Format:	Journal Article
Language:	English
Published:	United States AAAS 01-08-2018 American Association for the Advancement of Science
Subjects:	BASIC BIOLOGICAL SCIENCES SciAdv r-articles Scientific Community Synthetic Biology
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Abstract	Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing <US$100 per 30-person classroom. The user-friendly resources of this kit promise to enhance biology education both inside and outside the classroom.
AbstractList	Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing <US$100 per 30-person classroom. The user-friendly resources of this kit promise to enhance biology education both inside and outside the classroom. We report portable, user-friendly reagents and equipment for visual, hands-on biology activities with supporting curriculum. Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing <US$100 per 30-person classroom. The user-friendly resources of this kit promise to enhance biology education both inside and outside the classroom.
Author	Ottman, Sujud Granito, Teresa Qaq, Deema Gonzalzles, Eddie Marie Collins, James J Hill, Lander R Miller-Berry, Ashlee Mucha, Quinn Patel, Richa Chellaswamy, Jemima F Jewett, Michael C Gong, Elissa Stark, Jessica C Donghia, Nina Faheem, Nuhie Koritsaris, Jenna Burke, Julie Palffy, Christina Patel, Angela Dubner, Rachel S Anderson, Mary Hsu, Karen J Ferrante, Thomas C Woods, TaRhonda Zondor, Tyler Puppala, Aparna Reichert, Kara Fetherling, Suzanne Pardee, Keith Huang, Ally Nguyen, Binh Packett, Jessica S Patel, Palak Schmid, Miriam Slaton, Adriane Kanapskyte, Ada Martinez, Thomas Skweres, Sheila Nguyen, Peter Q Brand, Lance
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Glenbard East High School, 1014 South Main Street, Lombard, IL 60148-3938, USA – sequence: 17 givenname: Ashlee surname: Miller-Berry fullname: Miller-Berry, Ashlee organization: Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA – sequence: 18 givenname: Aparna surname: Puppala fullname: Puppala, Aparna organization: Glenbrook South High School, 4000 West Lake Avenue, Glenview, IL 60026-1239, USA – sequence: 19 givenname: Kara orcidid: 0000-0002-7292-008X surname: Reichert fullname: Reichert, Kara organization: Jones College Prep High School, 700 South State Street, Chicago, IL 60605-2109, USA – sequence: 20 givenname: Miriam surname: Schmid fullname: Schmid, Miriam organization: Gwendolyn Brooks College Preparatory Academy, 250 East 111th Street, Chicago, IL 60628-4324, USA – sequence: 21 givenname: Lance orcidid: 0000-0001-5684-8607 surname: Brand fullname: Brand, Lance organization: Delta High School, 3400 East SR 28, Muncie, IN 47303, USA – sequence: 22 givenname: Lander R orcidid: 0000-0001-6411-2739 surname: Hill fullname: Hill, Lander R organization: ASPIRA Business and Finance High School, 2989 North Milwaukee Avenue, Chicago, IL 60618-7347, USA – sequence: 23 givenname: Jemima F orcidid: 0000-0003-3670-5785 surname: Chellaswamy fullname: Chellaswamy, Jemima F organization: Aptakisic-Tripp School District 102, 850 Highland Grove Drive, Buffalo Grove, IL 60089, USA – sequence: 24 givenname: Nuhie orcidid: 0000-0003-0725-5666 surname: Faheem fullname: Faheem, Nuhie organization: Oak Lawn Hometown Middle School, 5345 West 99th Street, Oak Lawn, IL 60453-3815, USA – sequence: 25 givenname: Suzanne surname: Fetherling fullname: Fetherling, Suzanne organization: Hoffman Estates High School, 1100 West Higgins Road, Hoffman Estates, IL 60169-4050, USA – sequence: 26 givenname: Elissa surname: Gong fullname: Gong, Elissa organization: Vernon Hills High School, 145 Lakeview Parkway, Vernon Hills, IL 60061-1566, USA – sequence: 27 givenname: Eddie Marie surname: Gonzalzles fullname: Gonzalzles, Eddie Marie organization: Morgan Park High School, 1744 West Pryor Avenue, Chicago, IL 60643-3497, USA – sequence: 28 givenname: Teresa surname: Granito fullname: Granito, Teresa organization: Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA – sequence: 29 givenname: Jenna surname: Koritsaris fullname: Koritsaris, Jenna organization: Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA – sequence: 30 givenname: Binh surname: Nguyen fullname: Nguyen, Binh organization: Northside College Prep High School, 5501 North Kedzie Avenue, Chicago, IL 60625-3923, USA – sequence: 31 givenname: Sujud orcidid: 0000-0003-0243-3822 surname: Ottman fullname: Ottman, Sujud organization: Aqsa School, 7361 West 92nd Street, Bridgeview, IL 60455-2133, USA – sequence: 32 givenname: Christina surname: Palffy fullname: Palffy, Christina organization: Adlai E. Stevenson High School, 1 Stevenson Drive, Lincolnshire, IL 60069-2824, USA – sequence: 33 givenname: Angela surname: Patel fullname: Patel, Angela organization: Lyons Township High School, 100 South Brainard Avenue, La Grange, IL 60525-2101, USA – sequence: 34 givenname: Sheila surname: Skweres fullname: Skweres, Sheila organization: Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA – sequence: 35 givenname: Adriane orcidid: 0000-0002-2916-4186 surname: Slaton fullname: Slaton, Adriane organization: Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA – sequence: 36 givenname: TaRhonda surname: Woods fullname: Woods, TaRhonda organization: Evanston Township High School, 1600 Dodge Avenue, Evanston, IL 60201-3449, USA – sequence: 37 givenname: Nina orcidid: 0000-0003-0826-904X surname: Donghia fullname: Donghia, Nina organization: Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA – sequence: 38 givenname: Keith orcidid: 0000-0002-3812-8013 surname: Pardee fullname: Pardee, Keith organization: Leslie Dan Faculty of Pharmacy, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada – sequence: 39 givenname: James J orcidid: 0000-0002-5560-8246 surname: Collins fullname: Collins, James J organization: Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA – sequence: 40 givenname: Michael C orcidid: 0000-0003-2948-6211 surname: Jewett fullname: Jewett, Michael C organization: Simpson Querrey Institute, Northwestern University, 303 East Superior Street, Suite 11-131, Chicago, IL 60611-2875, USA
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ContentType	Journal Article
Copyright	Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 2018 The Authors
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Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 USDOE Office of Science (SC), Biological and Environmental Research (BER) SC0018249 These authors contributed equally to this work. Present address: Ombudsman Chicago South CPS Options School, 6057 South Western Avenue, Chicago, IL 60636–2019, USA.
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Snippet	Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However,... We report portable, user-friendly reagents and equipment for visual, hands-on biology activities with supporting curriculum. Synthetic biology offers...
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Title	BioBits™ Bright: A fluorescent synthetic biology education kit
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