Effect of Dynorphin A (1-17) on Proliferation and IL-2, IL-4, IFN-γ Production by Peripheral Blood Mononuclears

• Published in: Bulletin of experimental biology and medicine Vol. 150; no. 1; pp. 48 - 52
• Main Authors: Gein, S. V, Siytchihin, A. A
• Format: Journal Article
• Language: English
• Published: Boston Boston : Springer US 01-12-2010; Springer US; Springer
• Subjects: Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Proliferation - drug effects; Cells, Cultured; dynorphin A; Dynorphins - pharmacology; Humans; IL-2; IL-4; Interferon; Interferon-gamma - metabolism; Interleukin-2 - metabolism; Interleukin-4 - metabolism; Internal Medicine; Laboratory Medicine; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Mitogens; opiate receptors; Pathology; Peptides; Pharmacology and Toxicology; proliferation; IL-4; IL-2; proliferation; opiate receptors; dynorphin A
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-010-1065-2

Dynorphin A (1-17) in concentrations of 10⁻⁸-10⁻⁹ M inhibits phytohemagglutinin (2.5 μg/ml)-induced proliferative response of mononuclear fraction lymphocytes. In mitogen-stimulated cultures, 10⁻⁸ M dynorphin A (1-17) stimulates the production of IL-4, inhibits the production of IL-2, and does not modify the production of IFN-γ. Nonselective κ-receptor antagonist naloxone and selective antagonist binaltorphimine hydrochloride abolish the inhibitory effects of both dynorphin A concentrations on the lymphocyte proliferative response. On the other hand, evaluation of the effect of κ-receptor blockade on the production of IL-2 and IL-4 showed that this effect depends on peptide concentration and antagonist type. Hence, the results attest to an important role of κ-receptors in modulation of functional activity of immune cells.