Human Alveolar Macrophages May Not Be Susceptible to Direct Infection by a Human Influenza Virus

The current studies were undertaken to determine the susceptibility of human alveolar macrophages (AMs) to influenza A virus (IAV) infection in comparison with autologous peripheral blood-derived monocytes-macrophages (PBMs). AMs and PBMs were exposed to IAV in vitro and examined for their ability t...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases Vol. 214; no. 11; pp. 1658 - 1665
Main Authors: Ettensohn, David B., Frampton, Mark W., Nichols, Joan E., Roberts, Norbert J.
Format: Journal Article
Language:English
Published: United States Oxford University Press 01-12-2016
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The current studies were undertaken to determine the susceptibility of human alveolar macrophages (AMs) to influenza A virus (IAV) infection in comparison with autologous peripheral blood-derived monocytes-macrophages (PBMs). AMs and PBMs were exposed to IAV in vitro and examined for their ability to bind and internalize IAV, and synthesize viral proteins and RNA. PBMs but not AMs demonstrated binding and internalization of the virus, synthesizing viral proteins and RNA. Exposure of AMs in the presence of a sialidase inhibitor or anti-IAV antibody resulted in viral protein synthesis by the cells. Exposure of AMs to fluorescein isothiocyanate-labeled IAV in the presence of anti-fluorescein isothiocyanate antibody also resulted in viral protein synthesis. Thus, human AMs are apparently not susceptible to direct infection by a human IAV but are likely to be infected indirectly in the setting of exposure in the presence of antibody that binds the challenging strain of IAV.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Present affiliation: Department of Medicine, Warren Alpert Medical School of Brown University, Providence, Rhode Island.
D. B. E., M. W. F., and J. E. N. contributed equally to this work.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jiw413