Rapid isolation of DNA from Actinomyces

DNA could not be quickly extracted from members of the genus Actinomyces by the usual methods of lysis. Treatment of 7 different actinomyces cells with lysozyme and achromopeptidase, both 5 mg/g wet cells, for 2 h, followed by SDS (0.2%), proteinase K (5 mg/g wet cells) and EDTA (lmM) for 1 h, lysed...

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Bibliographic Details
Published in:	Annales de l'Institut Pasteur. Microbiology Vol. 138; no. 5; p. 529
Main Authors:	Barsotti, O, Renaud, F, Freney, J, Benay, G, Decoret, D, Dumont, J
Format:	Journal Article
Language:	English
Published:	France 01-09-1987
Subjects:	Actinomyces - genetics DNA, Bacterial - analysis DNA, Bacterial - isolation & purification Edetic Acid - metabolism Electrophoresis, Agar Gel Endopeptidase K Muramidase - metabolism Serine Endopeptidases - metabolism Sodium Dodecyl Sulfate - metabolism
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Summary:	DNA could not be quickly extracted from members of the genus Actinomyces by the usual methods of lysis. Treatment of 7 different actinomyces cells with lysozyme and achromopeptidase, both 5 mg/g wet cells, for 2 h, followed by SDS (0.2%), proteinase K (5 mg/g wet cells) and EDTA (lmM) for 1 h, lysed the cells. The yield obtained in one day was 337 micrograms per 200 mg of bacterial cells. The treatment was also found to work effectively on strains belonging to Veillonella, Staphylococcus, Fusobacterium and Bifidobacterium genera.
ISSN:	0769-2609
DOI:	10.1016/0769-2609(87)90038-X