Expression in rat fibroblasts of a human transforming growth factor-alpha cDNA results in transformation
Acquisition of the ability to produce and respond to a growth factor may result in increased cellular proliferation and could lead to malignant transformation. The fact that a large variety of tumor cells secrete transforming growth factor-alpha (TGF-alpha) suggests involvement of TGF-alpha in cellu...
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| Published in: | Cell Vol. 46; no. 2; p. 301 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
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United States
18-07-1986
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| Abstract | Acquisition of the ability to produce and respond to a growth factor may result in increased cellular proliferation and could lead to malignant transformation. The fact that a large variety of tumor cells secrete transforming growth factor-alpha (TGF-alpha) suggests involvement of TGF-alpha in cellular transformation and provides supporting evidence for the autocrine stimulation model. In order to determine directly the role of TGF-alpha in tumorigenicity, we introduced a human TGF-alpha cDNA expression vector into established nontransformed Fischer rat fibroblast (Rat-1) cells. Synthesis and secretion of human TGF-alpha by these cells results in the loss of anchorage-dependent growth and induces tumor formation in nude mice. Anti-human TGF-alpha monoclonal antibodies prevent TGF-alpha expressing Rat-1 cells from forming colonies in soft agar. |
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| AbstractList | Acquisition of the ability to produce and respond to a growth factor may result in increased cellular proliferation and could lead to malignant transformation. The fact that a large variety of tumor cells secrete transforming growth factor-alpha (TGF-alpha) suggests involvement of TGF-alpha in cellular transformation and provides supporting evidence for the autocrine stimulation model. In order to determine directly the role of TGF-alpha in tumorigenicity, we introduced a human TGF-alpha cDNA expression vector into established nontransformed Fischer rat fibroblast (Rat-1) cells. Synthesis and secretion of human TGF-alpha by these cells results in the loss of anchorage-dependent growth and induces tumor formation in nude mice. Anti-human TGF-alpha monoclonal antibodies prevent TGF-alpha expressing Rat-1 cells from forming colonies in soft agar. |
| Author | Lindquist, P B Derynck, R Rosenthal, A Bringman, T S Goeddel, D V |
| Author_xml | – sequence: 1 givenname: A surname: Rosenthal fullname: Rosenthal, A – sequence: 2 givenname: P B surname: Lindquist fullname: Lindquist, P B – sequence: 3 givenname: T S surname: Bringman fullname: Bringman, T S – sequence: 4 givenname: D V surname: Goeddel fullname: Goeddel, D V – sequence: 5 givenname: R surname: Derynck fullname: Derynck, R |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/3459590$$D View this record in MEDLINE/PubMed |
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| SubjectTerms | Animals Cell Adhesion Cell Transformation, Neoplastic - metabolism DNA - metabolism Female Fibroblasts - metabolism Growth Substances - physiology Humans Mice Mice, Inbred BALB C Mice, Nude Neoplasm Proteins - physiology Peptides - metabolism Peptides - physiology Phenotype Rats Transforming Growth Factors |
| Title | Expression in rat fibroblasts of a human transforming growth factor-alpha cDNA results in transformation |
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