Antigenic determinants at the carboxy terminus of chicken egg white riboflavin carrier protein (RCP): epitope mapping and antibody-mediated pregnancy curtailment in rodents

The epitopic core sequences recognized by three monoclonal antibodies raised to chicken riboflavin carrier protein (RCP) were mapped to the C-terminal tail-end of the protein using the pepscan method. A 21-residue synthetic peptide corresponding to residues 200–219 of the protein and comprising the...

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Bibliographic Details
Published in:	Vaccine Vol. 14; no. 4; pp. 307 - 312
Main Authors:	Koshy, Beena T., Karande, Anjali A., Adiga, P.Radhakantha
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-03-1996 Elsevier
Subjects:	Amino Acid Sequence Animals Antibodies - immunology Antibodies - pharmacology Antibody Specificity antipeptide antibodies Biological and medical sciences Birth control Carrier Proteins - analysis Carrier Proteins - immunology Chicken riboflavin carrier protein Chickens Contraception, Immunologic Epitope Mapping epitopes Epitopes - analysis Epitopes - immunology Female Gynecology. Andrology. Obstetrics Induced abortion. Therapeutic abortion inhibition of pregnancy establishment Medical sciences Membrane Transport Proteins Mice Molecular Sequence Data Peptide Fragments - immunology Pregnancy Rabbits Rats Rats, Wistar inhibition of pregnancy establishment epitopes Chicken riboflavin carrier protein antipeptide antibodies Antigenic determinant C terminal peptide Antibody Abortifacient Animal Riboflavin Abortion Structure function relationship Carrier protein
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Summary:	The epitopic core sequences recognized by three monoclonal antibodies raised to chicken riboflavin carrier protein (RCP) were mapped to the C-terminal tail-end of the protein using the pepscan method. A 21-residue synthetic peptide corresponding to residues 200–219 of the protein and comprising the regions corresponding to the antibodies was synthesized. Administration of polyclonal antibodies specific to this peptide led to termination of early pregnancy in mice. Also, active immunization of rats with the peptide-purified protein derivative conjugate inhibited establishment of pregnancy. These results demonstrate the functional importance of the C-terminal 200–219 region of chicken RCP.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0264-410X 1873-2518
DOI:	10.1016/0264-410X(95)00199-B