Immunocytochemical localization of glutamate dehydrogenase in mitochondria of the cerebellum: an ultrastructural study using a monoclonal antibody

Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degrees of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and...

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Bibliographic Details
Published in:	Brain research Vol. 452; no. 1-2; p. 396
Main Authors:	Madl, J E, Clements, J R, Beitz, A J, Wenthold, R J, Larson, A A
Format:	Journal Article
Language:	English
Published:	Netherlands 14-06-1988
Subjects:	Animals Antibodies, Monoclonal Cells, Cultured Cerebellum - enzymology Cerebellum - ultrastructure Glutamate Dehydrogenase - analysis Immunohistochemistry Microscopy, Electron Mitochondria - enzymology Rats
Online Access:	Get more information
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Summary:	Monoclonal antibodies to glutamate dehydrogenase (GDH) were produced and shown to have high degrees of specificity using immunoblots and ELISA. Immunocytochemical staining of electron microscopic preparations revealed selective intense staining of mitochondria in Bergmann glia, oligodendrocytes and astrocytes in the cerebellum of the rat. Differential intensity of staining among mitochondria within individual glial cells and between glial cells was observed and may provide an anatomical means of detecting differences in glutamate metabolism.
ISSN:	0006-8993
DOI:	10.1016/0006-8993(88)90047-9