Differential reactivity of Agaricus bisporus lectin with human IgA subclasses in gel precipitation

Differential reactivity of Agaricus bisporus lectin with human IgA subclasses in gel precipitation

The interaction between purified Agaricus bisporus lectin and several human proteins was studied using the Ouchterlony double diffusion and immunoelectrophoresis techniques. Only one precipitation line was observed with normal human serum, normal human colostrum, IgA1 myeloma serum, both serum monoc...

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Bibliographic Details
Published in:Journal of immunological methods Vol. 156; no. 2; p. 199
Main Authors: Irazoqui, F J, Zalazar, F E, Chiabrando, G A, Romero, O, Vides, M A
Format: Journal Article
Language:English
Published: Netherlands 08-12-1992
Subjects:
Humans
Immunodiffusion
Immunoelectrophoresis
Immunoglobulin A - chemistry
Immunoglobulin Isotypes - chemistry
In Vitro Techniques
Lectins - chemistry
Phytohemagglutinins - chemistry
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Summary:The interaction between purified Agaricus bisporus lectin and several human proteins was studied using the Ouchterlony double diffusion and immunoelectrophoresis techniques. Only one precipitation line was observed with normal human serum, normal human colostrum, IgA1 myeloma serum, both serum monoclonal and secretory IgA1 and monoclonal IgD. No reaction was observed with monoclonal and secretory IgA2, IgG, IgM, alpha 2 macroglobulin or pregnancy-associated alpha 2 glycoprotein. These results were confirmed by hemagglutination inhibition assays when IgA1, IgA2 and IgD were tested. On the basis of this reactivity, ABL could be a useful tool for distinguishing and isolating human IgA subclasses.
ISSN:0022-1759
DOI:10.1016/0022-1759(92)90026-P