Evaluation of murine lymphocyte membrane potential, intracellular free calcium, and interleukin-2 receptor expression upon exposure to 1,1-dimethylhydrazine

• Published in: Toxicology letters Vol. 61; no. 1; p. 27
• Main Authors: Frazier, Jr, D E, Tarr, M J, Olsen, R G
• Format: Journal Article
• Language: English
• Published: Netherlands 01-06-1992
• Subjects: Animals; Calcium - metabolism; Cell Division - drug effects; Concanavalin A - pharmacology; Dimethylhydrazines - pharmacology; Female; Flow Cytometry; Lymphocyte Activation - drug effects; Lymphocytes - drug effects; Lymphocytes - metabolism; Membrane Potentials; Mice; Mice, Inbred BALB C; Receptors, Interleukin-2 - drug effects; Receptors, Interleukin-2 - metabolism; Spleen - cytology; Thymus Gland - cytology; Time Factors
• ISSN: 0378-4274
• DOI: 10.1016/0378-4274(92)90060-W

The effects of 1,1-dimethylhydrazine (UDMH) on several early events associated with lymphocyte activation were examined. The concentration of intracellular calcium ([Ca2+]i) and membrane potential of murine lymphocytes were found to be altered upon exposure to UDMH; [Ca2+]i was increased in murine thymocytes, while splenocytes exhibited membrane hyperpolarization. In addition, interleukin-2 receptor expression induced by in-vitro concanavalin A stimulation of murine splenocytes at 24 and 48 h in the presence of UDMH was not affected. UDMH may interfere with the ability of these two distinct lymphocyte populations to regulate normal ionic fluctuations, thus contributing to altered immune responsiveness.