The Rhizobium leucaenae CFN 299 pSym plasmid contains genes expressed in free life and symbiosis, as well as two replication systems

The Rhizobium leucaenae CFN 299 pSym plasmid contains genes expressed in free life and symbiosis, as well as two replication systems

The alpha-protobacterium Rhizobium leucaenae CFN 299 is able to nodulate and fix nitrogen in symbiosis with a wide range of legumes, including Phaseolus vulgaris (common bean). Strain CFN 299 contains a 500-kb symbiotic plasmid (pSym) that encodes genes required for nodulation and nitrogen fixation...

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Bibliographic Details
Published in:Annals of microbiology Vol. 67; no. 3; pp. 263 - 273
Main Authors: Gamez-Reyes, Aurora, Becerra-Lobato, Noé, Ramírez-Trujillo, José Augusto, Martínez-Romero, Esperanza, Dunn, Michael F., Hernández-Lucas, Ismael
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2017
Springer
Springer Nature B.V
Subjects:
Applied Microbiology
Backup software
Beans
Biomedical and Life Sciences
Cell culture
Conjugation
DNA biosynthesis
DNA replication
Fixation
Genetic aspects
Genetic transcription
Legumes
Life Sciences
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Mimosaceae
Mycology
Neomycin
Nitrogen
Nitrogen fixation
Nodulation
Nodules
Original Article
Phaseolus vulgaris
Physiological aspects
Replication
Sulfur
Sulfur compounds
Symbiosis
Transcription
Transposition
Nodulation
Replication systems
CFN 299
Symbiotic plasmid
Gene expression
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Description
Summary:The alpha-protobacterium Rhizobium leucaenae CFN 299 is able to nodulate and fix nitrogen in symbiosis with a wide range of legumes, including Phaseolus vulgaris (common bean). Strain CFN 299 contains a 500-kb symbiotic plasmid (pSym) that encodes genes required for nodulation and nitrogen fixation as well as many genes whose function is unknown. In this work, we characterized the transcriptional expression of 16 pSym genes in common bean nodules and in free-living cells grown in culture. A functionally diverse group of genes were expressed during discrete stages of the symbiosis or in free-living cells. These included genes whose products are involved in nodulation and nitrogen fixation, carbon metabolism, vitamin synthesis, sulfur utilization, conjugation, transposition and DNA replication. We also examined the functionality of two replication systems encoded on pSym and found that repABC , but not repC2 , is required for pSym replication.
ISSN:1590-4261
1869-2044
DOI:10.1007/s13213-017-1257-3