Development of a real time polymerase chain reaction assay for equine encephalosis virus

•The S7 genes of 38 EEV isolates were sequenced.•A real-time RT-PCR assay for equine encephalosis virus was developed.•The 95% limit of detection of the EEV assay was 102.9 TCID50.•The assay was specific for EEV and did not detect other orbiviruses (BTV, AHSV). Equine encephalosis virus (EEV) is the...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 195; pp. 205 - 210
Main Authors:	Rathogwa, N.M., Quan, M., Smit, J.Q., Lourens, C., Guthrie, A.J., van Vuuren, M.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-01-2014
Subjects:	Animals Diagnostic assay DNA Primers - genetics Equine encephalosis virus Group-specific Horse Diseases - diagnosis Horse Diseases - virology Horses Orbivirus Orbivirus - classification Orbivirus - genetics Orbivirus - isolation & purification Real time RT-PCR Real-Time Polymerase Chain Reaction - methods Reoviridae Infections - diagnosis Reoviridae Infections - veterinary Reoviridae Infections - virology Sensitivity and Specificity Sequence Analysis, DNA Veterinary Medicine - methods Viral Proteins - genetics Virology - methods VP7 Group-specific Diagnostic assay VP7 Real time RT-PCR Equine encephalosis virus Orbivirus
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