Estrous cycle contributes to state-dependent contextual fear in female rats

• Published in: Psychoneuroendocrinology Vol. 141; p. 105776
• Main Authors: Blair, R. Shelby, Acca, Gillian M., Tsao, Barbara, Stevens, Naomi, Maren, Stephen, Nagaya, Naomi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-07-2022
• Subjects: Animals; Conditioning, Classical; Diestrus; Estrogens - pharmacology; Estrous Cycle; Fear; Fear conditioning; Female; Humans; Male; Ovariectomy; Proestrus; Progesterone; Progesterone - metabolism; Rats; State-dependent learning; Progesterone; Ovariectomy; Diestrus; State-dependent learning; Proestrus; Fear conditioning
• DOI: 10.1016/j.psyneuen.2022.105776
• EISSN: 1873-3360
• ISSN: 0306-4530

The increased susceptibility of women to stress and trauma-related disorders compared to men suggests a role for ovarian hormones in modulating fear and anxiety. In both humans and rodents, estrogen and progesterone have been shown to influence fear learning during acquisition, expression, and extinction. Recently, we showed that allopregnanolone (ALLO), a progesterone (PROG) metabolite and GABAA receptor potentiator, confers state-dependent contextual fear when infused into the bed nucleus of the stria terminalis of male rats. In order to determine whether estrous cycle-related fluctuations in circulating PROG confer state-dependent contextual fear in female rats, animals received Pavlovian fear conditioning during an estrous cycle phase when PROG was either low (late diestrus) or high (late proestrus). After conditioning, animals were tested for contextual fear in either the same or different estrous cycle phase. Subjects conditioned in diestrus and tested in proestrus showed lower levels of contextual fear compared to subjects conditioned and tested in the same estrous cycle phase (either diestrus or proestrus), suggesting a state-dependent effect of estrous cycle phase on fear learning. This state dependence was asymmetric, however, as animals trained in proestrus and tested in diestrus exhibited high levels of contextual fear. In ovariectomized (OVX) females treated acutely with either PROG or vehicle, state dependence was not observed. These results suggest that the hormonal state in diestrus may play a role in conferring state dependence to conditioned fear in naturally cycling female rats but not in an OVX model.
•Estrous cycle phase is associated with state-dependent contextual fear.•State dependence is conferred by conditioning in diestrus but not in proestrus.•State dependence is not conferred by low or high progesterone in OVX females.•Consideration of hormonal state may impact effectiveness of exposure therapy.