Array biosensor based on enzyme kinetics monitoring by fluorescence spectroscopy: Application for neurotoxins detection

The aim of the present work is to develop an evanescence wave array biosensor exploiting the “kinetic” approach of enzymatic reaction and further detection of the reaction products via pH sensitive fluorophore reporter. To demonstrate the feasibility of this approach, we have developed a biosensor a...

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Bibliographic Details
Published in:	Biosensors & bioelectronics Vol. 22; no. 12; pp. 3001 - 3007
Main Authors:	Ramanathan, M., Simonian, A.L.
Format:	Journal Article
Language:	English
Published:	Lausanne Elsevier B.V 15-06-2007 Elsevier Science
Subjects:	Array biosensor Biological and medical sciences Biosensing Techniques - methods Biotechnology DFP Enzyme Stability Enzymes, Immobilized - metabolism Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Isoflurophate - analysis Kinetics Organophosphorus hydrolase Paraoxon Paraoxon - analysis Parathion Parathion - analysis Sensitivity and Specificity Spectrometry, Fluorescence - methods TiO2 Titanium Parathion Array biosensor Organophosphorus hydrolase Paraoxon DFP TiO2 Insecticide Enzyme Pesticides Esterases Fluorescence spectrometry Phosphoric triester hydrolases Toxin Biosensor Neurotoxin Hydrolases Organophosphorus compounds Aryldialkylphosphatase Kinetics Detection Sensor array Application Monitoring
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