Two-dimensional difference gel electrophoresis applied for analytical proteomics: fundamentals and applications to the study of plant proteomics

• Published in: Analyst (London) Vol. 136; no. 20; p. 4119
• Main Authors: Arruda, Sandra Cristina Capaldi, Barbosa, Herbert de Sousa, Azevedo, Ricardo Antunes, Arruda, Marco Aurélio Zezzi
• Format: Journal Article
• Language: English
• Published: England 21-10-2011
• Subjects: Electrophoresis, Gel, Two-Dimensional; Fluorescent Dyes - chemistry; Plants - metabolism; Proteome - analysis; Proteomics - methods
• ISSN: 1364-5528
• DOI: 10.1039/c1an15513j

The present review reports the principles, fundamentals and some applications of two-dimensional difference gel electrophoresis for analytical proteomics based on plant proteome analysis, also emphasizing some advantages of 2-D DIGE over 2-D PAGE techniques. Some fluorescent protein labeling reagents, methods of protein labeling, models of 2-D DIGE experiments, and some limitations of this technique are presented and discussed in terms of 2-D DIGE plant proteomes. Finally, some practical applications of this technique are pointed out, emphasizing its potentialities in plant proteomics.