Variability in Assembly of Degradation Operons for Naphthalene and its derivative, Carbaryl, Suggests Mobilization through Horizontal Gene Transfer

• Published in: Genes Vol. 10; no. 8; p. 569
• Main Authors: Phale, Prashant S, Shah, Bhavik A, Malhotra, Harshit
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-07-2019; MDPI
• Subjects: Adaptation; Bacteria - genetics; Bacteria - metabolism; Biodegradation; Biosphere; Carbaryl; Carbaryl - metabolism; Carbon; Carcinogens; Consortia; Dehydrogenases; Enzymes; Evolution; Evolution, Molecular; Food webs; Gene Expression Regulation, Bacterial; Gene transfer; Gene Transfer, Horizontal; Genes; Genomes; Genomic islands; Horizontal transfer; Metabolic pathways; Metabolism; Metabolites; Microorganisms; Mutagens; Naphthalene; Operon; Operons; Organisms; Pesticides; Phages; Phenotypes; Plasmids; Pollutants; Polymorphism, Genetic; Prophages; Review; Transposons; Xenobiotics; integrative conjugative elements; pathway assembly; Carbaryl; naphthalene; xenobiotics; mobile genetic elements; transposons; evolution; horizontal gene transfer
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes10080569

In the biosphere, the largest biological laboratory, increased anthropogenic activities have led microbes to evolve and adapt to the changes occurring in the environment. Compounds, specifically xenobiotics, released due to such activities persist in nature and undergo bio-magnification in the food web. Some of these compounds act as potent endocrine disrupters, mutagens or carcinogens, and therefore their removal from the environment is essential. Due to their persistence, microbial communities have evolved to metabolize them partially or completely. Diverse biochemical pathways have evolved or been assembled by exchange of genetic material (horizontal gene transfer) through various mobile genetic elements like conjugative and non-conjugative plasmids, transposons, phages and prophages, genomic islands and integrative conjugative elements. These elements provide an unlimited opportunity for genetic material to be exchanged across various genera, thus accelerating the evolution of a new xenobiotic degrading phenotype. In this article, we illustrate examples of the assembly of metabolic pathways involved in the degradation of naphthalene and its derivative, Carbaryl, which are speculated to have evolved or adapted through the above-mentioned processes.