Screening of δ-aminolevulinic acid dehydratase from Pseudomonas strains as biosensor for lead and some other metals contamination

• Published in: Environmental monitoring and assessment Vol. 134; no. 1-3; pp. 263 - 269
• Main Authors: Korcan, S. Elif, Ciğerci, İ. Hakki, Konuk, Muhsin
• Format: Journal Article
• Language: English
• Published: Dordrect Dordrecht : Springer Netherlands 01-11-2007; Springer
• Subjects: ALAD; Analysis methods; Applied sciences; Biological and medical sciences; Biosensing Techniques; Biosensor; Biosensors; Biotechnology; Earth sciences; Earth, ocean, space; Engineering and environment geology. Geothermics; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; lead; metals; Metals, Heavy - toxicity; Methods. Procedures. Technologies; Natural water pollution; Pollution; Pollution, environment geology; Porphobilinogen Synthase - metabolism; Pseudomonas; Pseudomonas - enzymology; Pseudomonas - isolation & purification; Pseudomonas aeruginosa; Pseudomonas pseudoalcaligenes; Pseudomonas putida; Rivers; Various methods and equipments; Water Pollutants - toxicity; Water treatment and pollution; Pseudomonadales; Pseudomonas pseudoalcaligenes; Lyases; Aminolevulinic acid; Citrobacter freundii; ALAD; Pollution; Enzymatic activity; Lead; Bacteria; Pseudomonadaceae; Dehydratase; Pseudomonas aeruginosa; Pseudomonas putida; Enterobacteriaceae; Metals, Pseudomonas; Enzyme; Metalloproteins; Heavy metal; Measuring instrument; Surveillance; Biosensor; Water pollution; Carbon-oxygen lyases; Hydro-lyases
• ISSN: 0167-6369; 1573-2959
• DOI: 10.1007/s10661-007-9615-4

δ-Aminolevulinic acid dehydratase (ALAD; E.C. 4.2.1.24) is a metalloprotein and plays a crucial role in heme synthesis. Its sensitivity to toxic metals depends on the metallic co-factors. In this study the effects of some heavy metals on ALADs activity of five Pseudomonas isolates from Akarçay stream (Afyonkarahisar) have been studied in order to determine whether their ALADs could be used as biosensor for lead and other heavy metals contamination. The data obtained from the study were analysed statistically by using SPSS 10.0 software for Windows. According to the results, Ni(II) increased the ALAD activity of Pseudomonas putida, Pseudomonas pseudoalcaligenes and Pseudomonas aureginosa ATTC 27853. Mn(II) also increased the enzymic activity in all strains examined except P. pseudoalcaligenes. These were found not to be statistically meaningful. P. aeruginosa 2's enzymic activity was inhibited by Mg(II) and Zn(II), significantly (p < 0.05). There was a statistically meaningful relation between enzymic activity of both P. pseudoalcaligenes, P. putida and increasing Pb(II) concentration (p < 0.05). In addition, a formula was also deviced in order to determine the doses of metals in the environment of the organisms. As a result of the study, we can suggest that Pseudomonas ALADs can be used as a biosensor for lead and some other heavy metal exposure in aquatic environments.