Stabilization of Butyrylcholinesterase by the Entrapment into the Natural Polymer-Based Gels

Stabilization of Butyrylcholinesterase by the Entrapment into the Natural Polymer-Based Gels

A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics Vol. 479; no. 1; pp. 98 - 100
Main Authors: Lonshakova-Mukina, V. I., Esimbekova, E. N., Kratasyuk, V. A.
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-03-2018
Springer Nature B.V
Subjects:
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Drying
Gelatin
Gels
Immobilization
Life Sciences
Molecular Biology
Organophosphorus pesticides
Pesticides
Starch
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Summary:A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE, which allowed us to simplify the procedure and reduce the time of analysis of organophosphorus pesticides. The resulting immobilized samples retained activity for at least 300 days. BuChE samples based on the starch gel showed a greater sensitivity in the determination of pesticides as compared to the samples based on the gelatin gel.
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ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672918020126