Production of omega-5 gliadin monoclonal antibodies for allergenic evaluation of WDEIA-causing wheat varieties

• Published in: Applied biological chemistry Vol. 66; no. 1; pp. 46 - 10
• Main Authors: Sim, Jae-Ryeong, Lee, Jong-Yeol, Kim, Sewon
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01-12-2023; Springer Nature B.V; SpringerOpen; 한국응용생명화학회
• Subjects: Allergens; Anaphylaxis; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Chromosomes; Cloning; Crop science; Disease; Electrophoresis; Flour; Gliadin; Gluten; Immunoblot analysis; Immunoblotting; Ingestion; Loam soils; Monoclonal antibodies; Monoclonal antibody; Omega-5 gliadins; Peptides; Proteins; Standard operating procedures (SOP); Triticum aestivum; Wheat; Wheat-dependent exercise-induced anaphylaxis (WDEIA); 농학; Monoclonal antibody; Omega-5 gliadins; Immunoblot analysis; Wheat; Wheat-dependent exercise-induced anaphylaxis (WDEIA); Standard operating procedures (SOP)
• ISSN: 2468-0842; 2468-0834; 2468-0842
• DOI: 10.1186/s13765-023-00807-0

In allergic individuals, ingestion of wheat can lead to wheat-dependent exercise-induced anaphylaxis (WDEIA). Many studies have been conducted to find WDEIA allergen–deficient wheat, including by generating omega-5 gliadin antibodies. However, the reported antibodies have not been specific enough to detect omega-5 gliadins encoded on the 1B chromosome. In this study, we generated monoclonal antibodies against the major allergens causing WDEIA, omega-5 gliadins. Using these antibodies (mono-O5B-1C10), we assessed accumulation of omega-5 gliadins in wild-type and nullisomic-tetrasomic (NT) lines of the wheat (
Triticum aestivum
) varieties Chinese Spring (CS) by one- and two-dimensional gel electrophoresis, followed by Coomassie blue staining or immunoblotting with mono-O5B-1C10. We also tested mono-O5B-1C10 for major omega-5 gliadins in various wheat germplasms. Our results thus demonstrate the specificity of mono-O5B-1C10 for major omega-5 gliadins and potentially useful for identifying of omega-5 gliadin–deficient wheat varieties that should not cause WDEIA.