Heparin binding proteins from Leishmania ( Viannia) braziliensis promastigotes

• Published in: Veterinary parasitology Vol. 145; no. 3; pp. 234 - 239
• Main Authors: Azevedo-Pereira, R.L., Pereira, M.C.S., Oliveria-Junior, F.O.R., Brazil, R.P., Côrtes, L.M.C., Madeira, M.F., Santos, A.L.S., Toma, L., Alves, C.R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30-04-2007
• Subjects: Animals; binding properties; binding proteins; Cell Adhesion Molecules - metabolism; erythrocytes; gut proteins; hemagglutination; Heparin; Hydrophobic proteins; insect proteins; insect vectors; Leishmania; Leishmania ( V.) braziliensis; Leishmania braziliensis; Leishmania braziliensis - metabolism; Lutzomyia; Lutzomyia intermedia; Lutzomyia whitmani; Promastigotes; Protozoan Proteins - metabolism; Viannia braziliensis; Promastigotes; Hydrophobic proteins; Leishmania; Leishmania ( V.) braziliensis; Heparin; Lutzomyia intermedia; Lutzomyia whitmani
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2006.12.019

We have examined the heparin binding proteins from
Leishmania (
Viannia)
braziliensis promastigotes (HBP-Lb) by chromatography assays. The proposed strategy to isolate an enriched fraction of the HBP-Lb consisted of an association of the Triton X-114 method with affinity chromatography in heparin-Sepharose 4B column. SDS-PAGE analysis of the eluted proteins showed two main protein bands (65.0 and 54.5
kDa), while a single protein band was observed in native electrophoresis gel. The hemagglutination property of HBP-Lb over rabbit erythrocytes was confirmed up to 6.3
±
0.5
μg of protein mL
−1. Additionally, we have assayed the potential of HBP-Lb labeled with sulfo-NHS-LC-biotin in binding to nitrocellulose-immobilized gut proteins extracted of
Lutzomyia intermedia and
Lutzomyia whitmani. The results indicated a similar profile of five ligands (67.0, 62.1, 59.5, 56.0 and 47.5
kDa) in both studied
Lutzomyia species. This is the first direct description of this class of protein in
L. (
V.)
braziliensis with a suggestion of its biological activity in the interaction of
Leishmania with
Lutzomyia gut cells, which maybe a crucial step during this parasite's life cycle.