Synthesis and characterization of bovine femur bone hydroxyapatite containing silver nanoparticles for the biomedical applications

• Published in: Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology Vol. 13; no. 5; pp. 1917 - 1927
• Main Authors: Nirmala, R., Sheikh, Faheem A., Kanjwal, Muzafar A., Lee, John Hwa, Park, Soo-Jin, Navamathavan, R., Kim, Hak Yong
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-05-2011; Springer Nature B.V
• Subjects: Bacteria; Bones; Characterization and Evaluation of Materials; Chemical properties; Chemistry and Materials Science; Crystals; E coli; Hydroxyapatite; Inorganic Chemistry; Lasers; Materials Science; Nanoparticles; Nanotechnology; Optical Devices; Optics; Photonics; Physical Chemistry; Research Paper; Scanning electron microscopy; Silver; Surgical implants; Thermal decomposition; Transmission electron microscopy; X-ray diffraction; Reduction process; Thermal decomposition; Bactericidal; Pharmaceutical product; Hydroxyapatite; Ag nanoparticles; Nanomedicine
• ISSN: 1388-0764; 1572-896X
• DOI: 10.1007/s11051-010-9944-z

Bovine femur bone hydroxyapatite (HA) containing silver (Ag) nanoparticles was synthesized by thermal decomposition method and subsequent reduction of silver nitrate with
N
,
N
-dimethylformamide (DMF) in the presence of poly(vinylacetate) (PVAc). The structural, morphological, and chemical properties of the HA–Ag nanoparticles were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), field-emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). TEM images showed that the Ag nanoparticles with size ranging from 8 to 20 nm and were arranged at the periphery of HA crystals. Bactericidal activity of HA–Ag with different concentration of Ag nanoparticles immobilized on the surface of HA was investigated against gram-positive
Staphylococcus aureus
(
S. aureus
, non-MRSA), Methicillin resistant
S. aureus
(MRSA) and gram-negative
Escherichia coli
(
E.
coli
) by the disc diffusion susceptibility test. The HA–Ag nanoparticles showed that broad spectrum activity against non-MRSA, MRSA, and
E. coli
bacterial strains.