Identification and Cytogenetic Analysis of an Abnormal Pig Chromosome for Flow Cytometry and Sorting

• Published in: Zeitschrift für Naturforschung C. A journal of biosciences Vol. 48; no. 7; pp. 645 - 653
• Main Authors: Hausmann, Michael, Popescu, C. Paul, Boscher, Jeannine, Kerboœf, Dominique, Dölle, Jürgen, Cremer, Christoph
• Format: Journal Article
• Language: English
• Published: Tübingen Verlag der Zeitschrift für Naturforschung 01-08-1993
• Subjects: Animals; Banding of Flow Sorted Chromosomes; Biological and medical sciences; Cell Line; Chromatin. Chromosome; Chromosome Aberrations; Chromosome Banding; Chromosome Disorders; Chromosome Mapping; Fibroblasts - cytology; Flow Cytometry - methods; Flow Karyotyping; Fundamental and applied biological sciences. Psychology; Karyotyping; Molecular and cellular biology; Molecular genetics; Pig Chromosomes; Slit-Scan Flow Cytometry; Swine - genetics; Translocation Chromosomes; Translocation, Genetic; Chromosomal aberration; Flow cytometry; Chromosome; Sorting; Vertebrata; Mammalia; Swine; Cytogenetics; Abnormal chromosome; Artiodactyla; Ungulata; Karyotype; Chromosome translocation
• ISSN: 0939-5075; 1865-7125
• DOI: 10.1515/znc-1993-7-819

For cytogenetics of pig (Sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to estab­lish DNA-libraries, or to produce DNA-probes. Flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are described. Flow sorting of the largest chromosomes of these cells was performed. After sorting the chromosomes still had a well preserved morphology and were identified microscopically by G-banding. The quality of the band pattern of the sorted chromosomes was compatible to that of isolated chromosomes not subjected to flow cytometry. The sorted fraction showed an enrichment of chromosom e 6/15 and chromosome 1 which have quantitatively about the same integrated fluorescence intensity. Slit scan flow analysis was performed to discriminate these two chromosomes. Metacentric and submetacentric chromosom es were analyzed according to their bimodal slit scan profiles. Profiles of the largest chromosomes were distinguished by their different centromeric indices. Two groups were interpreted as the normal chromosome 1 and the translocation chromosom e 6/15.