Association of interleukin-2 and interferon-γ single nucleotide polymorphisms with Juvenile systemic lupus erythematosus

• Published in: Allergologia et immunopathologia Vol. 44; no. 5; p. 422
• Main Authors: Harsini, S, Ziaee, V, Tahghighi, F, Mahmoudi, M, Rezaei, A, Soltani, S, Sadr, M, Moradinejad, M H, Aghighi, Y, Rezaei, N
• Format: Journal Article
• Language: English
• Published: Spain 01-09-2016
• Subjects: Case-Control Studies; Child; DNA Mutational Analysis; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Interferon-gamma - genetics; Interleukin-2 - genetics; Iran; Lupus Erythematosus, Systemic - genetics; Polymorphism, Single Nucleotide; Interferon-gamma; Interleukin-2; Systemic lupus erythematosus; Children; Single nucleotide polymorphism
• ISSN: 1578-1267
• DOI: 10.1016/j.aller.2015.12.005

Juvenile systemic lupus erythematosus (JSLE) is a severe and chronic autoimmune disease of unknown origin. Inflammatory cytokines can play a pivotal role in the pathogenesis of JSLE, while their secretion is under genetic control. The current investigation was performed to analyse the associations of particular single nucleotide polymorphisms (SNPs) of interleukin-2 (IL-2) and interferon-gamma (IFN-γ) genes in a case control study.
The allele, genotype and haplotype frequencies of the polymorphic IL-2 (G/T at -330, rs2069762, and G/T at +166, rs2069763) and IFN-γ (A/T at +874, rs2430561) genes were estimated in 59 patients with JSLE by contrast with 140 healthy controls using polymerase chain reaction with sequence-specific primers method.
Results of the analysed data revealed a negative allelic association for JSLE in IL-2 -330/T (P=0.02), as well as a positive allelic association for IL-2 -330/G (P=0.02). IL-2 GG genotype (-330) in the patient group was also significantly overrepresented (P<0.001), while IL-2 GT genotype (-330) was notably decreased in the patients with JSLE (P<0.001). Additionally, the frequency of IL-2 (-330, +166) GT haplotype was significantly higher in the patient group (P<0.001).
IL-2 cytokine gene polymorphisms could affect individual susceptibility to JSLE and can take on the role of possible genetic markers for vulnerability to JSLE.