Isolation and characterization of a new immortal rat astrocyte with a high expression of NGF mRNA

We have established a new line of immortalized rat astrocytes through transfection of plasmid pSV3-neo encoding the large T antigen of simian virus 40 into normal astrocytes. One of these immortalized astrocytes (ACT-57) with a flat and polygonal cell shape, exhibited stable growth in a chemically d...

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Bibliographic Details
Published in:	Neuroscience research Vol. 39; no. 2; pp. 205 - 212
Main Authors:	Morikawa, Masayuki, Asai, Kiyofumi, Kokubo, Minoru, Fujita, Kaori, Yoneda, Kazuhiro, Yamamoto, Naoki, Inoue, Yuichiro, Iida, Junzo, Kishimoto, Toshifumi, Kato, Taiji
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 01-02-2001
Subjects:	Animals Astrocyte Astrocytes - cytology Astrocytes - metabolism Brain - cytology Brain - metabolism Brain-Derived Neurotrophic Factor - genetics Cell Culture Techniques - methods Cells, Cultured Embryo, Mammalian Glial fibrillary acidic protein Immortalization Karyotyping Nerve growth factor Nerve Growth Factor - genetics Neurotrophin 3 - genetics Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics SV40 T antigen Transcription, Genetic SV40 T antigen Nerve growth factor Astrocyte Immortalization Glial fibrillary acidic protein
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